Abstract
Haploid technology is used to develop uniform, true-breeding lines, as well as to accelerate crop improvement programs. Among 20 Apiaceae species screened for response to doubled haploidy, 11 generated microspore-derived embryos, and all but one of the latter yielded doubled haploid plants. Donor plant conditions, basal media, and culture conditions were evaluated for their efficacy on inducing microspore-derived embryos. Growing donor plants at temperature conditions of 10/5 or 15/10°C promoted microspore embryogenesis in fennel (Foeniculum vulgare Mill.), whereas, growing donor plants at a temperature regime of 10/5°C, along with the use of a cold extraction method, enhanced embryogenesis in dill (Anethum graveolens L.) and anise (Pimpinella anisum L.). The culture of fennel and dill microspores in an NLN basal medium and caraway (Carum carvi L.) in AT-3 basal medium promoted the highest frequencies of embryo induction.
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The authors thank Shelley Eftoda for her technical assistance and Karen Caswell, Patricia Polowick, and Monika Lulsdorf for reviewing the manuscript.
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Ferrie, A.M.R., Bethune, T.D. & Mykytyshyn, M. Microspore embryogenesis in Apiaceae. Plant Cell Tiss Organ Cult 104, 399–406 (2011). https://doi.org/10.1007/s11240-010-9770-0
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DOI: https://doi.org/10.1007/s11240-010-9770-0