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RETRACTED ARTICLE: In vitro propagation of Canscora decussata Schult. and comparative assessment of anti-cholinesterase and antioxidant capacities of wild-harnessed and in vitro-grown plant extracts

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This article was retracted on 25 July 2019

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Abstract

Canscora decussata Schult., popularly known as ‘shankhpushpi’, is a threatened medicinal plant. The whole plant is used in Ayurveda for curing insanity, epilepsy and nervous debility. Seasonal and limited availability along with the demand in herbal industries justify the need for developing in vitro plantlets for sustainable utilization of this plant. In the present study, an efficient protocol for in vitro propagation of C. decussata has been developed. Highest shoot induction was observed in the Murashige and Skoog (MS) basal medium supplemented with 0.2 mg l−1 benzylaminopurine (BAP) when nodal explants were used. Enhanced shoot induction (83.34 %) and multiplication (3.85 ± 0.23 average shoots per explant) were achieved when in vitro grown nodal buds were cultured on MS medium containing optimized BAP along with 1 mg l−1 naphthaleneacetic acid. Different solvent extracts of in vitro grown plantlets and wild plants were compared for acetylcholinesterase (AChE) inhibitory and antioxidant capacities. Highest AChE inhibitory activity was observed in ethanolic extract of wild plant biomass (IC50, 31.70 ± 0.16 µg ml−1) followed by ethanolic extract of in vitro plantlets (IC50, 44.17 ± 0.42 µg ml−1). On a whole, extracts of wild plant as well as in vitro plantlets exhibited the ability to scavenge DPPH and ABTS radicals, reduce ferric iron and protect DNA. These findings suggest that in vitro plantlets of C. decussata represent a promising alternative source for the botanicals ‘sankhapushpi’ for alleviating nervous ailments.

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Change history

  • 25 July 2019

    The editor has retracted this article [1] because a scientific error was made in the identification of the species referred to in the study. The plant studied is not Canscora decussata, as stated in the manuscript, but rather Canscora diffusa (Vahl) R.Br. ex Roem. & Schult. Because of this unintentional error, the scientific content of the article is no longer reliable. All authors agree to this retraction.

  • 25 July 2019

    The editor has retracted this article [1] because a scientific error was made in the identification of the species referred to in the study. The plant studied is not Canscora decussata, as stated in the manuscript, but rather Canscora diffusa (Vahl) R.Br. ex Roem. & Schult. Because of this unintentional error, the scientific content of the article is no longer reliable. All authors agree to this retraction.

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Acknowledgments

NKG thanks Ministry of Human Resource Development (MHRD) for the award of an institute research assistantship. Facilities created from an MHRD-sponsored research grant (4-25/2013/TS-I) were utilized in this work.

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Authors and Affiliations

  1. Natural Product Biotechnology Group, Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur, 721 302, India

    Nitin Kundlikrao Gaikwad, Utkarsh Ravindra Moon & Adinpunya Mitra

  2. Soil Science and Plant Nutrition Laboratory, Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur, 721 302, India

    Nitin Kundlikrao Gaikwad & Pratapbhanu Singh Bhadoria

Authors
  1. Nitin Kundlikrao Gaikwad
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  2. Utkarsh Ravindra Moon
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  3. Pratapbhanu Singh Bhadoria
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  4. Adinpunya Mitra
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Corresponding author

Correspondence to Adinpunya Mitra.

Additional information

The editor has retracted this article [1] because a scientific error was made in the identification of the species referred to in the study. The plant studied is not Canscora decussata, as stated in the manuscript, but rather Canscora diffusa (Vahl) R.Br. ex Roem. & Schult. Because of this unintentional error, the scientific content of the article is no longer reliable. All authors agree to this retraction.

[1] Gaikwad NK, MoonUR Bhadoria PS Mitra A (2015) In vitro propagation of Canscora decussata Schult. and comparative assessment of anti-cholinesterase and antioxidant capacities of wild-harnessed and in vitro-grown plant extracts. Plant Cell Tiss Organ Cult. 122: 509-516. doi:10.1007/s11240-015-0770-y

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Gaikwad, N.K., Moon, U.R., Bhadoria, P.S. et al. RETRACTED ARTICLE: In vitro propagation of Canscora decussata Schult. and comparative assessment of anti-cholinesterase and antioxidant capacities of wild-harnessed and in vitro-grown plant extracts. Plant Cell Tiss Organ Cult 122, 509–516 (2015). https://doi.org/10.1007/s11240-015-0770-y

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  • DOI: https://doi.org/10.1007/s11240-015-0770-y

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