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Functional characterization of the pathogenesis-related protein family 10 gene, PgPR10-4, from Panax ginseng in response to environmental stresses

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Abstract

Pathogenesis-related proteins (PRs) are known to function in higher plants as a protein-based defensive system against abiotic and biotic stress, particularly pathogen infections. A full-length cDNA sequence of PR BetV1 was isolated and characterized from a 14-year-old ginseng expressed sequence tags library and we named this as PgPR10-4, because of similar identities with previous isolated PgPR10s sequences. The PgPR10-4 gene encodes a 477 bp open reading frame and its deduced protein contains 158 amino acids with a 53 % identity with that of the Actinidia chinensis BetV1 allergen. The expression of PgPR10-4 gene was abundant in leaves and its transcripts showed differentially up-regulated patterns against several ginseng pathogens and abiotic stimuli such as high light and salinity. In addition, PgPR10-4 expression was strongly responsive towards the stress signaling molecules H2O2 and jasmonic acid (JA), while weekly responsive to salicylic acid and abscisic acid. A functional role of PgPR10-4 in environmental stress tolerance was further validated through its overexpression in Arabidopsis. An analysis of T2 transgenic Arabidopsis plants overexpressing the PgPR10-4 gene showed an enhanced tolerance to bacterial and fungal infection, but not to salt stress. When we tagged with cyan fluorescent protein fusion protein, the PgPR10-4-was found to localize to the cytoplasm. The enhanced antifungal activity observed from the Arabidopsis transgenic lines suggests the possible involvement of PgPR10-4 in a defense-related mechanism via the JA signaling pathway.

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Abbreviations

cDNA:

Complementary DNA

EST:

Expressed sequence tag

ORF:

Open reading frame

PR:

Pathogenesis-related

qRT-PCR:

Quantitative reverse transcription-polymerase chain reaction

H2O2 :

Hydrogen peroxide

JA:

Jasmonic acid

SA:

Salicylic acid

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Acknowledgments

This research was supported by iPET (112142-05-1-CG000), Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries, Republic of Korea.

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  1. Department of Oriental Medicinal Materials and Processing, College of Life Science, Kyung Hee University, Suwon, 449-701, Korea

    Yu-Jin Kim, Moon-Gi Jang, Hye-Jin Lee, Gyu-Hwan Jang, Johan Sukweenadhi, Woo-Saeng Kwon & Deok-Chun Yang

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  1. Yu-Jin Kim
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11240_2014_505_MOESM1_ESM.docx

Nucleotide and deduced amino acid sequence of PgPR10-4 isolated from P. ginseng. The deduced amino acid sequence is shown as a single-letter code below the nucleotide sequence. The position of the nucleotides is shown on the right. Two black boxes show the transcription start codon (ATG) and termination codon (TAA), respectively. (DOCX 135 kb)

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Kim, YJ., Jang, MG., Lee, HJ. et al. Functional characterization of the pathogenesis-related protein family 10 gene, PgPR10-4, from Panax ginseng in response to environmental stresses. Plant Cell Tiss Organ Cult 118, 531–543 (2014). https://doi.org/10.1007/s11240-014-0505-5

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