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Enhanced production of isoflavones by elicitation in hairy root cultures of Soybean

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Abstract

A combined treatment of sonication (2 min) and vacuum infiltration (2 min) stimulated isoflavones production of 75.26 mg g−1 DW which was 15.11-fold higher than control hairy root line at optimal harvest time of 40 days. Addition of MeJ at 100 μM concentration with 72 h exposure time on 30 day-old hairy root culture further enhanced total isoflavones production of 53.16 mg g−1 DW (10.67-fold) and SA at 200 μM concentration with 96 h exposure period enhanced the production of isoflavones (28.79 mg g−1 DW; 5.78-fold). MeJ-treated hairy roots reduced biomass accumulation whereas sonication, vacuum infiltration and SA did not exhibit a negative effect on biomass growth.

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Acknowledgments

The authors are thankful to the Department of Science and Technology (DST), Government of India, for the financial support (SERB/SR/SO/PS/85/2012 Dated 3.9.2013) to carry out the present work. Prof. A. Ganapathi is grateful to the University Grants Commissions, Government of India, for the award of UGC-BSR Fellowship. The first author thankfully acknowledges the UGC, Government of India, for the award of UGC-MANF.

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Authors and Affiliations

  1. Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India

    Jeevaraj Theboral, Ganeshan Sivanandhan, Kondeti Subramanyam, Markandan Manickavasagam & Andy Ganapathi

  2. Department of Genetic Engineering, School of Bioengineering, SRM University, Kattankulathur, Kancheepuram District, 603 203, Tamil Nadu, India

    Muthukrishnan Arun

  3. Plant Tissue Culture Laboratory, Department of Botany, Periyar E.V.R College, Tiruchirappalli, 620 023, Tamil Nadu, India

    Natesan Selvaraj

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  1. Jeevaraj Theboral
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  2. Ganeshan Sivanandhan
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  3. Kondeti Subramanyam
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  4. Muthukrishnan Arun
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  5. Natesan Selvaraj
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  6. Markandan Manickavasagam
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  7. Andy Ganapathi
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Correspondence to Andy Ganapathi.

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Theboral, J., Sivanandhan, G., Subramanyam, K. et al. Enhanced production of isoflavones by elicitation in hairy root cultures of Soybean. Plant Cell Tiss Organ Cult 117, 477–481 (2014). https://doi.org/10.1007/s11240-014-0450-3

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