Abstract
Phytosulfokines (PSKs) were the first peptidyl growth factors from a higher plant to be characterized. They induce the proliferation in suspension culture of mesophyll cells of asparagus (Asparagus officinalis L.; Matsubayashi and Sakagami 1996b). We examined the effects of PSK-α on the formation of adventitious roots on cucumber hypocotyls. The number of roots increased 2- to 2.5-fold after treatment with PSK-α but derivatives of PSK-α were inactive in our assay system. The effect of PSK-α on the formation of adventitious roots was also examined in terms of the expression of a newly isolated gene for a root-specific cystatin of cucumber (RCC). The results indicate that PSK-α can promote organogenesis in plant, acting possibly via enhancement of the proliferation of plant cells.
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Abbreviations
- CM:
-
conditioned medium
- PSK:
-
phytosulfokine
- RCC:
-
root-specific cystatin of cucumber
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The nucleotide sequence data reported in this paper will appear in the GSDB, DDBJ, EMBL, and NCBI nucleotide sequence databases with the following accession number AB014760 (RCC).
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Yamakawa, S., Sakuta, C., Matsubayashi, Y. et al. The promotive effects of a peptidyl plant growth factor, phytosulfokine-α, on the formation of adventitious roots and expression of a gene for a root-specific cystatin in cucumber hypocotyls. J. Plant Res. 111, 453–458 (1998). https://doi.org/10.1007/BF02507810
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DOI: https://doi.org/10.1007/BF02507810