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Cloning and characterization of a Doritaenopsis hybrid PRP39 gene involved in flowering time

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Abstract

The Arabidopsis thaliana PRP39, encoding a tetratricopeptide repeat protein, is a novel gene that affects flowering time via an autonomous pathway. In this study, a PRP39 homolog gene was isolated from Doritaenopsis ‘Tinny Tender’ (Doritaenopsis Happy smile × Happy valentine) and designated as DhPRP39. The full-length DhPRP39 cDNA was 2,962 bp long with a 2,472 bp open reading frame and encodes 823 amino acids. The putative DhPRP39-encoded protein contained four conserved, three half-a-tetratricopeptide repeat, and one RNA14 regions. Amino acid sequence alignment showed that DhPRP39 shares a high similarity to PRP39 homologs from other species. Real-time quantitative reverse transcription polymerase chain reaction analysis showed that DhPRP39 is ubiquitously expressed in vegetative and reproductive organs. The transcripts of this gene reached higher levels in the vegetative organs (roots, stems, and leaves) during the transition from vegetative to reproductive growth, in which the stems exhibited the strongest expression. DhPRP39 was overexpressed in Arabidopsis thaliana, and flowering of these transgenic plants were much delayed by 6–7 days compared with wild-type Arabidopsis. DhPRP39 may play an important role in the regulation of plant flowering.

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Abbreviations

CaMV:

Cauliflower mosaic virus

gDNA:

Genomic DNA

HAT:

Half-a-TPR

RACE:

Rapid amplification of cDNA ends

ORF:

Open reading frame

PPR:

Pentatricopeptide repeat

SSH:

Suppression subtractive hybridization

TPR:

Tetratricopeptide repeat

WT:

Wild-type

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (Grant Nos. 30771762 and 31170658) and the Zhejiang Provincial Natural Science Foundation of China (Grant No. Y3090532).

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Authors and Affiliations

  1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Lin’an, Hangzhou, 311300, Zhejiang, China

    Xiaoming Sun, Mingbing Zhou & Yongyi Cui

  2. School of Landscape Architecture, School of Tourism and Health, Zhejiang Agriculture and Forestry University, Lin’an, Hangzhou, 311300, Zhejiang, China

    Xiaoming Sun & Jing Zhang

  3. School of Agriculture and Food Science, Zhejiang Agriculture and Forestry University, Lin’an, Hangzhou, 311300, Zhejiang, China

    Qiaoping Qin, Chi Zhang & Yongyi Cui

  4. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, 361-763, Korea

    Kee Yoeup Paek

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  1. Xiaoming Sun
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  2. Qiaoping Qin
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  3. Jing Zhang
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  4. Chi Zhang
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  7. Yongyi Cui
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Correspondence to Yongyi Cui.

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Sun, X., Qin, Q., Zhang, J. et al. Cloning and characterization of a Doritaenopsis hybrid PRP39 gene involved in flowering time. Plant Cell Tiss Organ Cult 110, 347–357 (2012). https://doi.org/10.1007/s11240-012-0156-3

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  • DOI: https://doi.org/10.1007/s11240-012-0156-3

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