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Cool-night temperature induces spike emergence and affects photosynthetic efficiency and metabolizable carbohydrate and organic acid pools in Phalaenopsis aphrodite

  • Physiology and Biochemistry
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Abstract

After being acclimated to constant warm (28°C day/28°C night) and cool-night temperature (28°C day/20°C night) regimes in growth chambers for 2 weeks, the two groups of mature Phalaenopsis aphrodite subsp. formosana plants both clearly exhibited a diurnal oscillation of stomatal conductance, net CO2 uptake rate, malate and starch levels, and the phosphoenolpyruvate carboxylase (EC 4.1.1.31) and NAD+-malic enzyme (EC 1.1.1.39) activities. Hence, P. aphrodite is an obligate crassulacean acid metabolism plant. Nevertheless, different night temperature greatly affected both the stomatal conductance and the contribution of ambient and respiratory CO2 to the nocturnal accumulation of malate. However, the amounts of nocturnal accumulated malate and daily deposited starch appeared to have no significant difference between the two groups. These results demonstrate that P. ahrodite is congruent with the characteristics of CAM plants having great flexibility and plasticity in response to changes in environmental conditions. In addition, the formation of reproductive stem, viz. spike, was noticeably inhibited by a constant warm temperature, but induced by a fluctuating warm day and cool night condition. The relationship between the metabolic pool variation and spike induction of Phalaenopsis is also discussed.

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Abbreviations

CAM:

Crassulacean acid metabolism

NAD(P)+-ME:

NAD(P)+-malic enzyme

PEPCase:

Phosphoenolpyruvate carboxylase

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Acknowledgments

We sincerely thank Taiwan Sugar Research Institute for lending the LI-6400 (Li-Cor Inc., Lincoln NE, USA) and Professor Yau-Lun Kuo (Graduate Institute of Bioresources, National Pingtung University of Science and Technology) for providing a light emitting diode source (6400-02, Li-Cor Inc., Lincoln NE, USA) for gas exchange measurements. This work was supported by the grants 93AS-1.1.2-FD-Z3, 94AS-1.3.2-FD-Z3, and 95AS-1.3.2-FD-Z3 from the Council of Agriculture, Executive Yuan, Taiwan.

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Authors and Affiliations

  1. Department of Life Sciences, National University of Kaohsiung, No. 700 Kaohsiung University Rd, Nan-Tzu District 811, Kaohsiung, Taiwan, ROC

    Wen-Huei Chen, Yo-Ching Liu, Chuo-Min Chuo, Pai-Ting Chen, Kai-Meng Tseng, Yi-Chun Yeh, Mang-Jye Ger & Heng-Long Wang

  2. Institute of Biotechnology, National University of Kaohsiung, 811, Kaohsiung, Taiwan, ROC

    Wen-Huei Chen, Ya-Chen Tseng, Mang-Jye Ger & Heng-Long Wang

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  1. Wen-Huei Chen
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Correspondence to Heng-Long Wang.

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Communicated by W.-H. Wu.

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Chen, WH., Tseng, YC., Liu, YC. et al. Cool-night temperature induces spike emergence and affects photosynthetic efficiency and metabolizable carbohydrate and organic acid pools in Phalaenopsis aphrodite . Plant Cell Rep 27, 1667–1675 (2008). https://doi.org/10.1007/s00299-008-0591-0

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