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A combination of elicitation and precursor feeding leads to increased anthocyanin synthesis in cell suspension cultures of Vitis vinifera

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Abstract

Both elicitation and precursor feeding are effective strategies for improving secondary metabolite production in plant cell suspension cultures. In this study, cell suspension cultures of Vitis vinifera subjected to methyl jasmonate treatment resulted in a significant increase in levels of anthocyanin production. Moreover, a combination of 5 mg/L phenylalanine and 50 mg/L methyl jasmonate promoted the highest level of anthocyanin biosynthesis, resulting in 4.6- and 3.4-fold increases in anthocyanin content and yield, respectively, over the control. The optimum period for elicitation of anthocyanin synthesis was 4 days following incubation in the presence of elicitors, at the beginning of the exponential growth phase. V. vinifera cell lines of different anthocyanin-producing capabilities responded differently to elicitation and precursor feeding. Anthocyanin production of a low-producing cell line, VV06, could be enhanced with addition of elicitors and precursor feeding. Methyl jasmonate was the only elicitor that increased anthocyanin production of the high-producing cell line VV05, but contributed to moderate enhancement of anthocyanin production compared with VV06. For cell line VV06, synergistic effects were observed for all treatment combinations of methyl jasmonate along with other elicitors and precursors. In addition, 6.1- and 4.6-fold increases in anthocyanin content and yield, respectively, were obtained in the presence of 5 mg/L phenylalanine, 50 mg/L methyl jasmonate, and 1 mg/L dextran. However, none of these treatment combinations exhibited synergistic effects in cell line VV05.

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Abbreviations

NAA:

α-Naphthaleneacetic acid

K:

Kinetin

Me-JA:

Methyl jasmonate

FCW:

Fresh cell weight

DCW:

Dry cell weight

CV:

Color value

References

  • Aumont V, Larronde F, Richard T, Budzinski H, Decendit A, Deffieux G, Krisa S, Mérillon JM (2004) Production of highly 13C-labeled polyphenols in Vitis vinifera cell bioreactor cultures. J Biotechnol 109:287–294

    Article  PubMed  CAS  Google Scholar 

  • Bonfill M, Mangas S, Moyano E, Cusido RM, Palazón J (2011) Production of centellosides and phytosterols in cell suspension cultures of Centella asiatica. Plant Cell Tiss Organ Cult 104:61–67

    Article  CAS  Google Scholar 

  • Bourgaud F, Gravot A, Milesi S, Gontier E (2001) Production of plant secondary metabolites: a historical perspective. Plant Sci 161:839–851

    Article  CAS  Google Scholar 

  • Dai J, Patel JD, Mumper RJ (2007) Characterization of blackberry extract and its antiproliferative and anti-inflammatory properties. J Med Food 10(2):258–265

    Article  PubMed  CAS  Google Scholar 

  • Dass S, Ramawat KG (2009) Elicitation of guggulsterone production in cell cultures of Commiphora wightii by plant gums. Plant Cell Tiss Organ Cult 96:349–353

    Article  Google Scholar 

  • Dias LLC, Santa-Catarina C, Ribeiro DM, Barros RS, Floh EIS, Otoni WC (2009) Ethylene and polyamine production patterns during in vitro shoot organogenesis of two passion fruit species as affected by polyamines and their inhibitor. Plant Cell Tiss Organ Cult 99:199–208

    Article  CAS  Google Scholar 

  • Frankfater CR, Dowd MK, Triplett BA (2009) Effect of elicitors on the production of gossypol and methylated gossypol in cotton hairy roots. Plant Cell Tiss Organ Cult 98:341–349

    Article  CAS  Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–156

    Article  PubMed  CAS  Google Scholar 

  • Ketchum REB, Gibson DM, Croteau RB, Shuler ML (1999) The kinetics of taxoid accumulation in cell suspension cultures of Taxus following elicitation with methyl jasmonate. Biotechnol Bioeng 62:97–105

    Article  PubMed  CAS  Google Scholar 

  • Kim OT, Bang KH, Kim YC, Hyun DY, Kim MY, Cha SW (2009) Upregulation of ginsenoside and gene expression related to triterpene biosynthesis in ginseng hairy root cultures elicited by methyl jasmonate. Plant Cell Tiss Organ Cult 98:25–33

    Article  CAS  Google Scholar 

  • Kong JM, Chia LS, Goh NK, Chia TF, Brouillard R (2003) Analysis and biological activities of anthocyanins. Phytochemistry 64:923–933

    Article  PubMed  CAS  Google Scholar 

  • Korsangruang S, Soonthornchareonnon N, Chintapakorn Y, Saralamp P, Prathanturarug S (2010) Effects of abiotic and biotic elicitors on growth and isoflavonoid accumulation in Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension cultures. Plant Cell Tiss Organ Cult 103:333–342

    Article  CAS  Google Scholar 

  • Krisa S, Téguo PW, Decendit A, Deffieux JV, Mérillon JM (1999) Production of 13C-labelled anthocyanins by Vitis vinifera cell suspension cultures. Phytochemistry 51:651–656

    Article  PubMed  CAS  Google Scholar 

  • Qu JG, Zhang W, Yu XJ, Jin MF (2005) Instability of anthocyanin accumulation in Vitis vinifera L. var Gamay Fréaux suspension cultures. Biotechnol Bioprocess Eng 10:155–161

    Article  CAS  Google Scholar 

  • Qu JG, Zhang W, Jin MF, Yu XJ (2006) Effect of Homogeneity on Cell Growth and Anthocyanin Biosynthesis in Suspension Cultures of Vitis vinifera. Chin J Biotechnol 22(5):805–810

    Article  Google Scholar 

  • Rao SR, Ravishankar GA (2002) Plant cell cultures: chemical factories of secondary metabolites. Biotechnol Adv 20:101–153

    Article  PubMed  CAS  Google Scholar 

  • Rhee HS, Cho HY, Son SY, Yoon SYH, Park JM (2010) Enhanced accumulation of decursin and decursinol angelate in root cultures and intact roots of Angelica gigas Nakai following elicitation. Plant Cell Tiss Organ Cult 101:295–302

    Article  CAS  Google Scholar 

  • Roberts SC, Shuler ML (1997) Large-scale plant cell culture. Current Opinions Biotechnol 8:154–159

    Article  CAS  Google Scholar 

  • Sakuta M, Hirano H, Kakegawa K, Suda J, Hirose M, Joy RW IV, Sugiyama M, Komamine A (1994) Regulatory mechanisms of biosynthesis of betacyanin and anthocyanin in relation to cell division activity in suspension cultures. Plant Cell Tiss Org Cult 38:167–169

    Article  CAS  Google Scholar 

  • Springob K, Nakajima J, Yamazaki M, Saito K (2003) Recent advances in the biosynthesis and accumulation of anthocyanins. Nat Prod Rep 20:288–303

    Article  PubMed  CAS  Google Scholar 

  • Verpoorte R, Van der Heijden R, Schripsema J, Hoge JHC, Ten Hoopen HJG (1993) Plant cell biotechnology for the production of alkaloids: present status and prospects. J Nat Prod 56:186–207

    Article  CAS  Google Scholar 

  • Verpoorte R, Van der Heijden R, ten Hoopen HJG, Memelink J (1999) Metabolic engineering of plant secondary metabolite pathways for the production of fine chemicals. Biotechnol Lett 21:467–479

    Article  CAS  Google Scholar 

  • Whitmer S, Van der Heijden R, Verpoorte R (2002) Effect of precursor feeding on alkaloid accumulation by a tryptophan decarboxylase over-expressing transgenic cell line T22 of Catharanthus roseus. J Biotechnol 96:193–203

    Article  PubMed  CAS  Google Scholar 

  • Wiedenfeld H, Furmanowa M, Roeder E, Guzewska J, Gustowski W (1997) Camptothecin and 10-hydroxycamptothecin in callus and plantlets of Camptotheca acuminata. Plant Cell Tiss Org Cult 49:213–218

    Article  CAS  Google Scholar 

  • Wise ML, Sreenath HK, Skadsen RW, Kaeppler HK (2009) Biosynthesis of avenanthramides in suspension cultures of oat (Avena sativa). Plant Cell Tiss Organ Cult 97:81–90

    Article  CAS  Google Scholar 

  • Yuan YJ, Wei ZJ, Miao ZQ, Wu JC (2002) Acting paths of elicitors on Taxol biosynthesis pathway and their synergistic effect. Biochem Eng J 10:77–83

    Article  CAS  Google Scholar 

  • Zhang W, Furusaki S (1999) Production of anthocyanins by plant cell cultures. Biotechnol Bioprocess Eng 4:231–252

    Article  CAS  Google Scholar 

  • Zhang CH, Mei XG, Liu L, LJ YU (2000) Enhanced pacilitaxel production induced by the combination of elicitors in cell suspension cultures of Taxus chinensis. Biotechnol Lett 22:1561–1564

    Article  CAS  Google Scholar 

  • Zhang W, Curtin C, Kikuchi M, Franco C (2002) Integration of jasmonic acid and light irradiation for enhancement of anthocyanin biosynthesis in Vitis vinifera suspension cultures. Plant Sci 162:459–468

    Article  Google Scholar 

  • Zhong JJ (2002) Plant cell culture for production of paclitaxel and other taxanes. J Biosci Bioeng 94(6):591–599

    PubMed  CAS  Google Scholar 

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Acknowledgments

This research was financially supported by the National Natural Science Foundation of China (No. 20176058).

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Authors and Affiliations

  1. Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China

    Junge Qu, Wei Zhang & Xingju Yu

  2. Zhejiang Provincial Key Laboratory of Biopharmaceuticals Based in Ningbo, Zhejiang Pharmaceutical College, Ningbo, 315100, China

    Junge Qu

  3. CRC for Bioproducts and Department of Medical Biotechnology, School of Medicine, Flinders University, Adelaide, SA5042, Australia

    Wei Zhang

  4. Department of Applied Biology, Zhejiang Pharmaceutical College, Ningbo, 315100, China

    Junge Qu

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  1. Junge Qu
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  2. Wei Zhang
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Correspondence to Junge Qu.

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Qu, J., Zhang, W. & Yu, X. A combination of elicitation and precursor feeding leads to increased anthocyanin synthesis in cell suspension cultures of Vitis vinifera . Plant Cell Tiss Organ Cult 107, 261–269 (2011). https://doi.org/10.1007/s11240-011-9977-8

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  • DOI: https://doi.org/10.1007/s11240-011-9977-8

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