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Effects of exogenous nitric oxide on antioxidation and DNA methylation of Dendrobium huoshanense grown under drought stress

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Abstract

Nitric oxide (NO) is an important biological messenger in plants, which has been implicated in response to abiotic stress. To study the effects of exogenous NO on drought menace, the tube seedlings of Dendrobium huoshanense were selected and treated with 10% polyethylene glycol (PEG-6000) to simulate drought stress. After application of sodium nitroprusside (SNP), the relative water content (RWC) and antioxidant enzyme activities were determined. As a result, plant treated with 50 μmol L−1 of SNP maintained high level of RWC and lower content of malondialdehyde (MDA). Furthermore, the antioxidant enzyme activities were obviously enhanced. However, the higher concentration of SNP (100 μmol L−1) enhanced the effects of drought stress for plant. For further analysis of the response mechanism to exogenous NO, the methylation-sensitive amplified polymorphism (MSAP) technique was used to investigate the changes of DNA methylation. When the seedlings of Dendrobium huoshanense were treated with 50 μmol L−1 SNP containing 10% PEG-6000, levels of global DNA methylation of Dendrobium huoshanense were decreased. Nevertheless, the demethylation rate of methylated sites increased, accounting for 12.5% of total methylation sites. These results implied that some expressed genes were involved in the response process to drought stress triggered by NO in Dendrobium huoshanense.

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Abbreviations

NO:

Nitric oxide

SNP:

Sodium nitroprusside

PEG-6000:

Polyethylene glycol-6000

MSAP:

Methylation-sensitive amplified polymorphism

MS:

Murashige and Skoog

SOD:

Superoxide dismutase

POD:

Peroxidase

CAT:

Catalase

MDA:

Malondialdehyde

cPTIO:

2-(4-carboxyphenyl)-4,4,5,5,-tetramethy-limidazoline-1-oxyl-3-oxide

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Acknowledgments

The present investigation is financially supported by Anhui Provincial Natural Science Foundation for Distinguished Young Scholars (10040606Q22), the University Excellent Young Talents Foundation of Anhui Province (2011SQRL053) and Anhui agricultural University Young Talents Foundation (yj2008-27).

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Authors and Affiliations

  1. Department of Life Sciences, Anhui Agricultural University, Hefei, 230036, People’s Republic of China

    Honghong Fan, Tingchun Li, Lei Guan, Zhengpeng Li, Ning Guo, Yongping Cai & Yi Lin

  2. Anhui Academy of Agricultural Sciences, Hefei, 230031, People’s Republic of China

    Tingchun Li

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  1. Honghong Fan
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  2. Tingchun Li
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  3. Lei Guan
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  4. Zhengpeng Li
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  5. Ning Guo
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  6. Yongping Cai
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  7. Yi Lin
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Correspondence to Yi Lin.

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Honghong Fan and Tingchun Li contributed equally to the work.

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Fan, H., Li, T., Guan, L. et al. Effects of exogenous nitric oxide on antioxidation and DNA methylation of Dendrobium huoshanense grown under drought stress. Plant Cell Tiss Organ Cult 109, 307–314 (2012). https://doi.org/10.1007/s11240-011-0096-3

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  • DOI: https://doi.org/10.1007/s11240-011-0096-3

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