Skip to main content
SpringerLink
Log in

Factors influencing induction and maintenance of Vitis rotundifolia Michx. embryogenic cultures

  • Original Paper
  • Published:
Plant Cell, Tissue and Organ Culture (PCTOC) Aims and scope Submit manuscript

Abstract

Unopened leaves, petioles and fully opened leaves from micropropagation cultures of five Vitis rotundifolia Michx. varieties were cultured on induction medium to study their embryogenic response. Among the various explants tested, the maximum number of varieties produced embryogenic cultures from unopened leaves followed by fully opened leaves and petioles. Based on morphological differences, two types of embryogenic cultures were identified. Friable cultures typically arose as proembryonic masses (PEM) on induction medium, whereas somatic embryo production without an intervening PEM stage was observed in compact cultures. Of the five varieties tested, the highest frequency of embryogenic response was observed from fully opened leaves of ‘Supreme’ and unopened leaves and petioles of ‘Delicious’. Attempts to initiate suspension cultures from varieties resulted in proliferation and maintenance of ‘Alachua’ and ‘Carlos’ cultures in liquid medium for 16 weeks. Embryogenic potential of varieties was studied on cultures growing on embryo development medium. The maximum number of cotyledonary stage somatic embryos from 0.2 g proembryonic masses were observed in ‘Carlos’ (379.3) followed by ‘Alachua’ (350.0) and ‘Delicious’ (305.0). Cotyledonary stage somatic embryos germinated when cultured on Murashige and Skoog medium containing 1 μM Benzyladenine (BA). Although high embryo germination rates (80–100%) were observed in the varieties tested, plant recovery from germinated somatic embryos ranged from 6–47%. Embryogenic cultures could be maintained on X6 medium and used in genetic engineering studies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

BA:

Benzyladenine

MS:

Murashige and Skoog

NAA:

Naphthaleneacetic acid

References

  • Dhekney SA, Li ZT, Dutt M, Gray DJ (2008) Agrobacterium-mediated transformation of embryogenic cultures and regeneration of transgenic plants in Vitis routundifolia Michx. (muscadine grape). Plant Cell Rep 27:865–872

    Article  PubMed  CAS  Google Scholar 

  • Dhekney SA, Li ZT, Compton ME, Gray DJ (2009a) Optimizing initiation and maintenance of Vitis embryogenic cultures. HortScience 44:1400–1406

    Google Scholar 

  • Dhekney SA, Li ZT, Zimmerman TW, Gray DJ (2009b) Factors influencing genetic transformation and plant regeneration of Vitis. Amer J Enol Vitic 60:285–292

    CAS  Google Scholar 

  • Ector BJ, Magee JB, Hegwood CP et al (1996) Resveratrol concentration in muscadine berries, juice, pomace, purees, seeds and wine. Am J Enol Vitic 47:57–62

    CAS  Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirement of suspension cultures of soybean root cells. Exp Cell Res 50:151–158

    Article  PubMed  CAS  Google Scholar 

  • Gray DJ (1992) Somatic embryogenesis and plant regeneration from immature zygotic embryos of muscadine grape (Vitis rotundifolia) cultivars. Amer J Bot 79:542–546

    Article  Google Scholar 

  • Gray DJ (1995) Somatic embryogenesis in grape. In: Jain SM, Gupta PK, Newton RJ (eds) Somatic embryogenesis in woody plants, vol 2. Kluwer, Dordrecht, pp 191–217

    Google Scholar 

  • Gray DJ, Benton CM (1991) In vitro micropropagation and plant establishment of muscadine grape cultivars (Vitis rotundifolia). Plant Cell Tiss Org Cult 27:7–14

    Article  CAS  Google Scholar 

  • Gray DJ, Jayasankar S, Li Z (2005) Vitis spp grape, Chapt. 22. In: Litz RE (ed) Biotechnology of fruit and nut crops. Oxford CAB International Wallingford, Wallingford, pp 672–706

    Chapter  Google Scholar 

  • Himelrick DG (2003) Handling, storage and post-harvest physiology of muscadine grapes: a review. Small Fruits Rev 2:45

    Article  Google Scholar 

  • Jayasankar S, Gray DJ, Litz RE (1999) High frequency somatic embryogenesis and plant regeneration from suspension cultures of grapevine. Plant Cell Rep 18:533–537

    Article  CAS  Google Scholar 

  • Jayasankar S, Bondada BR, Li Z et al (2003) Comparative anatomy and morphology of Vitis vinifera (Vitaceae) somatic embryos from solid and liquid culture systems. Amer J Bot 90:973–979

    Article  Google Scholar 

  • Li ZT, Dhekney S, Dutt M et al (2006) Optimizing Agrobacterium-mediated transformation of grapevine. In Vitro Cell Dev Biol 42:220–227

    Article  CAS  Google Scholar 

  • Li ZT, Dhekney SA, Dutt M, Gray DJ (2008) An improved protocol for Agrobacterium-mediated transformation of grapevine (Vitis vinifera L.). Plant Cell Tissue Organ Cult 93:311–321

    Article  Google Scholar 

  • Merkle SA (1995) Somatic embryogenesis in Magnoliaceae (Liriodendron and Magnolia). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, somatic embryogenesis and synthetic seed I, vol 30. Springer, Berlin, pp 388–403

    Google Scholar 

  • Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163:85–87

    Article  PubMed  CAS  Google Scholar 

  • Patel GI, Olmo HP (1955) Cytogenetics of Vitis: I. the hybrid V. vinifera x V. rotundifolia. Amer J Bot 42:141–159

    Article  Google Scholar 

  • Robacker C (1993) Somatic embryogenesis and plant regeneration from muscadine grape leaf explants. Hort Science 28:53–55

    Google Scholar 

  • Stamp JA, Meredith CP (1988) Somatic embryogenesis from leaves and anthers of grapevine. Sci Hortic 35:235–250

    Article  Google Scholar 

  • Witjaksono, Litz RE (1999) Induction and growth characteristics of avocado embryogenic cultures. Plant Cell Tiss Org Cult 58:19–29

    Article  Google Scholar 

Download references

Acknowledgments

This research was supported by the Florida Department of Agriculture and Consumer Services’ Viticulture Trust Fund and the Florida Agricultural Experiment Station. We thank Mary S. Brennan for assistance with statistical analyses.

Author information

Authors and Affiliations

  1. Mid-Florida Research and Education Center, University of Florida/IFAS, 2725 Binion Road, Apopka, FL, 32703-8504, USA

    S. A. Dhekney, Z. T. Li & D. J. Gray

Authors
  1. S. A. Dhekney
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Z. T. Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. J. Gray
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to D. J. Gray.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dhekney, S.A., Li, Z.T. & Gray, D.J. Factors influencing induction and maintenance of Vitis rotundifolia Michx. embryogenic cultures. Plant Cell Tiss Organ Cult 105, 175–180 (2011). https://doi.org/10.1007/s11240-010-9849-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-010-9849-7

Keywords

Search

Navigation