Abstract
Drug yielding potential of turmeric (Curcuma longa L.) is due to the presence of important phytoconstituents such as curcumin, oleoresin and essential oil. Slow multiplication rate, high susceptibility to rhizome rot and leaf spot disease and restricted availability of elite genotype necessitated application of tissue culture technique to alleviate the problems. A protocol has been developed for in vitro micropropagation of an elite genotype (cv. suroma) using latent axillary bud explants from unsprouted rhizome, available throughout the year. MS media containing 3 mg/l 6-Benzyladenine (BA) and 1 mg/l Indole Acetic acid (IAA) was found optimum for regeneration, multiplication and in vitro conservation of plantlets. After 3 years of in vitro conservation regenerants were transplanted to field and assessed for drug yielding potential through evaluation of curcumin, oleoresin and essential oil contents of rhizomes and leaves. One year of field grown tissue culture derived turmeric were found uniform in all the characteristics examined, when compared with those grown conventionally. Micropropagated turmeric showing stable drug yielding potential also proved to have genetic basis of stability as revealed by RAPD based molecular profiling.
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Abbreviations
- BA:
-
6-Benzyladenine
- IAA:
-
Indole Acetic acid
- NAA:
-
Napthalene Acetic acid
- Kin:
-
Kinetin
- Ads:
-
Adenine Sulphate
- RAPD:
-
Random Amplified Polymorphic DNA
- MS:
-
Murashige and Skoog
- CD:
-
Critical differences
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Acknowledgments
The authors are grateful to Prof (Dr.) S.C. Si, Dean, Centre of Biotechnology and Prof (Dr.) M.R. Nayak, President, Siksha ‘O’ Anusandhan University for providing facilities and encouraging throughout.
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Singh, S., Kuanar, A., Mohanty, S. et al. Evaluation of phytomedicinal yield potential and molecular profiling of micropropagated and conventionally grown turmeric (Curcuma longa L.). Plant Cell Tiss Organ Cult 104, 263–269 (2011). https://doi.org/10.1007/s11240-010-9811-8
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DOI: https://doi.org/10.1007/s11240-010-9811-8