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Regeneration of transformed plants from hairy roots of Plumbago indica

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Abstract

Spontaneous shoot regeneration was observed from Agrobacterium rhizogenes-induced hairy roots of Plumbago indica when these were incubated in liquid MS medium for a period of 3 weeks under continuous light. Insertion of the rolB gene in putative transformed plants was confirmed by PCR and sequencing. Transformed plants grown for a period of 1 week on solid MS medium containing 0.5 mg l−1 6-benzyladenine and then transplanted to growth regulator-free medium showed better overall growth than control plants. Transformed plants had a higher root bio-biomass and an increased plumbagin content relative to non-transformed plants.

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Fig. 1
Fig. 2

Abbreviations

BA:

6-Benzyladenine

HPLC:

High performance liquid chromatography

IAA:

Indole acetic acid

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Acknowledgments

The authors thank the Director, Bose Institute for providing the necessary facilities and funding through an Institutional Fellowship to MG. The immensely helpful comments of the anonymous reviewers is thankfully acknowledged.

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Author notes

  1. Dipjyoti Chakraborty

    Present address: Department of Bioscience and Biotechnology, Bansthali Vidyapith, Banasthali, Rajasthan, 304022, India

Authors and Affiliations

  1. Medicinal Plant Laboratory, Department of Botany, Bose Institute, 93/1 APC Road, Kolkata, West Bengal, 700009, India

    Moumita Gangopadhyay & Sabita Bhattacharya

  2. Plant Molecular and Cellular Genetics, Bose Institute, Centenary Campus, P 1/12 CIT Road, Scheme VIIM, Kolkata, West Bengal, 700054, India

    Dipjyoti Chakraborty

  3. Department of Genetics, Bidhan Chandra Krishi Visyavidyalaya, Mohonpur, Nadia, West Bengal, 741252, India

    Somnath Bhattacharyya

Authors
  1. Moumita Gangopadhyay
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  2. Dipjyoti Chakraborty
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  3. Somnath Bhattacharyya
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  4. Sabita Bhattacharya
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Corresponding author

Correspondence to Moumita Gangopadhyay.

Electronic supplementary material

Below is the link to the electronic supplementary material.

11240_2010_9702_MOESM1_ESM.eps

Supplementary Fig. 1: HPLC chromatograms, a. standard plumbagin, b. transformed plant grown in 0.5 mg l−1 BA for 1 week, c. non-transformed plant. (EPS 589 kb)

Supplementary Fig. 2: Flowchart for transformation and spontaneous regeneration in P. indica. (DOC 27 kb)

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Gangopadhyay, M., Chakraborty, D., Bhattacharyya, S. et al. Regeneration of transformed plants from hairy roots of Plumbago indica . Plant Cell Tiss Organ Cult 102, 109–114 (2010). https://doi.org/10.1007/s11240-010-9702-z

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  • DOI: https://doi.org/10.1007/s11240-010-9702-z

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