Abstract
Rapid-cycling fast plants (Brassica rapa; RCBr) is also known as Wisconsin Fast Plant and is widely used in K-12 and undergraduate studies. RCBr has a short generation time (seed-to-seed in 30–60 days), which allows for the completion of experiments in a semester. Previous studies have shown that cotyledonary explants with attached petioles are capable of generating shoots. However, there is no published adventitious shoot regeneration protocol to date. Sterile cotyledonary explants were excised; all edges and petioles were removed. Five-day-old cotyledonary explants produced shoots on a Murashige and Skoog medium containing 1.5 mg/L thiadiazuron (TDZ) and 0.5 mg/L 1-naphthaleneacetic acid (NAA) (FPM I) at a mean rate of 8.8%. This rate increased to 14.8% in explants placed on FPM I medium supplemented with 5.0 mg/L silver nitrate (AgNO3) (SRM 2). The rate increased to 32.5% when 5-day-old explants, excised from the part of the cotyledon nearest to the petiole, were placed adaxial side up on SRM 2 medium. The shoot regeneration rate increased to 44.5% using 4-day-old cotyledonary explants. A shoot regeneration rate of 23% was observed among 9-day-old leaf explants. Shoots from cotyledonary explants were elongated on basal medium with 0.5 mg/L NAA, rooted on basal medium, and later acclimatized. This is the first report of shoot regeneration from cotyledonary explants of rapid-cycling Brassica rapa without pre-existing meristematic tissues.
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Acknowledgments
We would like to acknowledge receipt of funds from Dr. Ali A. Khan, Program Director of Mid-Eastern Alliance for Minority Participation (MEAMP) and Extramural Associates Research Development Award (EARDA); the Department of Education (Minority Science and Engineering Improvement Program: MSEIP); and the Department of Biology at ECSU for additional supplies and equipment. We would also like to thank Ms. Kaleena Green for her assistance on this project. This work was completed in partial fulfillment of Salimah Cogbill’s undergraduate biology senior honors thesis.
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Cogbill, S., Faulcon, T., Jones, G. et al. Adventitious shoot regeneration from cotyledonary explants of rapid-cycling fast plants of Brassica rapa L. Plant Cell Tiss Organ Cult 101, 127–133 (2010). https://doi.org/10.1007/s11240-010-9669-9
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DOI: https://doi.org/10.1007/s11240-010-9669-9