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Somatic hybrids obtained by asymmetric protoplast fusion between Musa Silk cv. Guoshanxiang (AAB) and Musa acuminata cv. Mas (AA)

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Abstract

To attempt to introduce genetic information of disease resistance from Musa acuminata cv. Mas (AA) to Musa silk cv. Guoshanxiang (AAB) and obtain somatic hybrids, we developed an asymmetric protoplast fusion with 20% (w/v) polyethylene glycol (PEG). The protoplasts derived from embryogenic suspension cultural cells of cv. Guoshanxiang (AAB) and cv. Mas (AA) were, respectively treated with 1.5 mM iodoacetamide (IOA) and with ultraviolet light (UV) at an intensity of 50 W/m2 for 120 s. A total of 47 regenerated green plants were obtained and eight of which were survived in greenhouse. Six of the survived plants were identified as hybrids by RAPD analysis and only three hybrids were retained vigorously in field. The hybrid nature of the three plants was further confirmed according to their ISSR (inter-simple sequence repeat) patterns and the results indicated that they were true somatic hybrids. Chromosome analysis revealed that the three hybrids possessed an aneuploid chromosome number (2n = 34).

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Abbreviations

BA:

6-Benzylaminopurine

2, 4-D:

2,4-Dichlorophenoxyacetic acid

ECS:

Embryogenic cell suspensions

IOA:

Iodoacetamide

ISSR:

Inter-simple sequence repeat

MES:

2-N-morpholino ethanesulfonic acid

NAA:

1-Naphthaleneacetic acid

PEG:

Polyethylene glycol

RAPD:

Random amplified polymorphism DNA

UV:

Ultraviolet light

References

  • Assani A, Haïcour R, Wenzel G, Côte FX, Bakry F, Foroughi-Wehr B, Ducreux G, Aguillar ME, Grapin A (2001) Plant regeneration from protoplasts of dessert banana cv. Grande Naine (Musa spp., Cavendish sub-group AAA) via somatic embryogenesis. Plant Cell Rep 20:482–488. doi:10.1007/s002990100366

    Article  CAS  Google Scholar 

  • Assani A, Haïcour R, Wenzel G, Foroughi-Wehr B, Bakry F, Côte FX, Ducreux G, Ambroise A, Grapin A (2002) Influence of donor material and genotype on protoplast regeneration in banana and plantain cultivars (Musa spp.). Plant Sci 162:355–362. doi:10.1016/S0168-9452(01)00562-3

    Article  CAS  Google Scholar 

  • Assani A, Chabane D, Haicour R, Bakry F, Wenzel G, Foroughi-Wehr B (2005) Protoplast fusion in banana (Musa spp.): comparison of chemical (PEG: polyethylene glycol) and electrical procedure. Plant cell Tiss Org Cul 83:145–151

    Article  CAS  Google Scholar 

  • Assani A, Chabane D, Foroughi-Wehr B, Wenzel G (2006) An improved protocol for microcallus production and whole plant regeneration from recalcitrant banana protoplasts (Musa spp.). Plant Cell Tissue Organ Cult 85:257–264. doi:10.1007/s11240-005-9058-y

    Article  Google Scholar 

  • Bauer-Weston B, Keller W, Webb J (1993) Production and characterization of asymmetric somatic hybrids between Arabidopsis thaliana and Brassica napus. Theor Appl Genet 86:150–158. doi:10.1007/BF00222073

    Article  Google Scholar 

  • Côte FX, Domergue R, Monmarson S, Schwendiman J, Teisson C, Escalant JV (1996) Embryogenic cell suspensions from the male flower of Musa AAA cv. Grande Naine. Physiol Plant 97:285–290. doi:10.1034/j.1399-3054.1996.970211.x

    Article  Google Scholar 

  • Du DL, Su J, Zhou P, Zheng XQ, Huang BZ, Li FN (2001) RAPD analysis of 33 varieties of banana. Acta Bot Sin 43:1036–1042

    CAS  Google Scholar 

  • Forsberg J, Dixelius C, Lagercrantz U, Glimelius K (1998) UV dose-dependent DNA elimination in asymmetric somatic hybrids between Brassica napus and Arabidopsis thaliana. Plant Sci 131:65–76. doi:10.1016/S0168-9452(97)00242-2

    Article  CAS  Google Scholar 

  • Huang BZ, Xu LB, Yang H, Tang XL, Wei YR, Qiu JS, Li GQ (2005) Preliminary results of field evaluation of banana germplasm resistant to Fusarium wilt disease. Guangdong Agric Sci 6:9–10 in Chinese with English abstract

    Google Scholar 

  • Kisaka H, Kisaka M, Kanno A, Kameya T (1998) Intergeneric somatic hybridization of rice (Oryza sativa L.) and barley (Hordeum vulgare L.) by protoplast fusion. Plant Cell Rep 17:362–367. doi:10.1007/s002990050407

    Article  CAS  Google Scholar 

  • Liu JH, Deng XX (1999) Regeneration of hybrid calluses via donor-recipient fusion between Microcitrus papuana and Citrus sinensis. Plant Cell Tissue Organ Cult 59:81–87. doi:10.1023/A:1006399302843

    Article  Google Scholar 

  • Matsumoto K, Oka S (1998) Plant regeneration from protoplasts of a Brazilian dessert banana (Musa spp., AAB group). Acta Hortic 490:455–462

    Google Scholar 

  • Matsumoto K, Vilarinhos AD, Oka S (2002) Somatic hybridization by electrofusion of banana protoplasts. Euphytica 125:317–324. doi:10.1023/A:1016071624090

    Article  CAS  Google Scholar 

  • Megia R, Hacour R, Tizroutine S, Bui Trang V, Rossignol L, Sihachakr D, Schwendiman J (1993) Plant regeneration from cultured protoplasts of the cooking banana cv. Bluggoe (Musa spp., ABB group). Plant Cell Rep 13:41–44. doi:10.1007/BF00232313

    Article  CAS  Google Scholar 

  • Morpurgo R, Lopato SV, Afza R, Novak FG (1994) Selection parameters for resistance to Fusarium oxysporum f.sp.Cubense race 1 and race 4 on diploid banana (Musa acuminata Colla). Euphytica 75:121–129. doi:10.1007/BF00024539

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497. doi:10.1111/j.1399-3054.1962.tb08052.x

    Article  CAS  Google Scholar 

  • Panis B, Wauwe AW, Swennen R (1993) Plant regeneration through direct somatic embryogenesis from protoplasts of banana (Musa spp.). Plant Cell Rep 12:403–407. doi:10.1007/BF00234701

    Article  Google Scholar 

  • Parokonny AS, Kenton A, Gleba YY, Bennett MD (1994) The fate of recombinant chromosomes and genome interaction in Nicotiana asymmetric somatic hybrids and their sexual progeny. Theor Appl Genet 89:488–497. doi:10.1007/BF00225385

    Article  CAS  Google Scholar 

  • Rasmussen JQ, Nepper JP, Rasmussen QS (1997) Regeneration and analysis of interspecific asymmetric potato-Solanum spp. hybrid plants selected by micromanipulation or fluorescence activated cell sorting (FACS). Theor Appl Genet 95:41–49. doi:10.1007/s001220050530

    Article  Google Scholar 

  • Spangenberg G, Valles MP, Wang ZY, Montavon P, Nagel J, Potrykus I (1994) Asymmetric somatic hybridization between tall fescue (Festuca arundinaceae Schreb) and irradiated Italian ryegrass (Lolium multiforum Lam) protoplast. Theor Appl Genet 88:509–519. doi:10.1007/BF01240911

    Article  Google Scholar 

  • Varotto S, Nenz E, Lucchin M, Parrini P (2001) Production of asymmetric somatic hybrid plants between Cichorium intybus L. and Helianthus annuus L. Theor Appl Genet 102:950–956. doi:10.1007/s001220000485

    Article  Google Scholar 

  • Vlahova M, Hinnisdales S, Frulleux F, Claeys M, Atanassov A, Jacobs M (1997) UV irradiation as a tool for obtaining asymmetric somatic hybrids between Nicotiana plumbaginigolia and Lycopersicon esculentum. Theor Appl Genet 94:184–191. doi:10.1007/s001220050398

    Article  Google Scholar 

  • Wang H, Xia GM, Chen HM (1994) Effect of UV on the protoplast division and chromosome variation of wheat. Acta Phytobiol Sin 4(1):69–72

    Google Scholar 

  • Wang YP, Sonntag K, Rudloff E (2003) Development of rapeseed with high erucic acid content by asymmetric somatic hybridization between Brassica napus and Crambe abyssinica. Theor Appl Genet 106:1147–1155

    PubMed  CAS  Google Scholar 

  • Wei YR, Huang XL, Huang X, Li J, Xiao W, Li XJ (2005a) The induction of multiple buds and somatic embryogenesis of Musa silk Guoshanxiang (AAB). Act Hortic Sin 32:414–419 in Chinese with English abstract

    CAS  Google Scholar 

  • Wei YR, Huang XL, Li J, Huang X, Li Z, Li XJ (2005b) Establishment of embryogenic cell suspension culture and plant regeneration of edible banana Musa acuminata cv. Mas (AA). Chin J Biotechnol 21:58–65 in Chinese with English abstract

    CAS  Google Scholar 

  • Xia GM, Chen HM (1996) Plant regeneration from intergeneric somatic hybridization between Triticum aestivum L. and Leymmus chinensis (trin) Tzvel. Plant Sci 120:197–203. doi:10.1016/S0168-9452(96)04492-5

    Article  CAS  Google Scholar 

  • Xia GM, Xiang FN, Zhou AF, Wang H, Chen HM (2003) Asymmetric somatic hybridization between wheat (Triticum aestivum L.) and Agropyron elongatum (Host) Nevishi. Theor Appl Genet 107:299–305. doi:10.1007/s00122-003-1247-7

    Article  PubMed  CAS  Google Scholar 

  • Xiang FN, Xia GM, Chen HM (2003) Effect of UV dosage on somatic hybridization between common wheat (Triticum aestivum) and Avena sativa L. Plant Sci 164:697–707. doi:10.1016/S0168-9452(03)00021-9

    Article  CAS  Google Scholar 

  • Xiao W, Huang XL, Huang X, Chen YP, Dai XM, Zhao JT (2007) Plant regeneration from protoplasts of Musa acaminata cv. Mas (AA) via somatic embryogenesis. Plant cell Tiss Org Cult 90:191–200

    Article  Google Scholar 

  • Xiao W, Huang X, Wei YR, Zhao JT, Dai XM, Huang XL (2008) Plant regeneration from protoplast culture of Musa AAB Silk cv. Guoshanxiang. Act Hortic Sin 35(6):873–878 in Chinese with English abstract

    Google Scholar 

  • Yamagishi H, Landgren M, Forsberg J, Glimelius K (2002) Production of asymmetric hybrids between Arabidopsis thaliana and Brassica nupus utilizing an efficient protoplast culture system. Theor Appl Genet 104:959–964. doi:10.1007/s00122-002-0881-9

    Article  PubMed  CAS  Google Scholar 

  • Yan CQ, Qian KX, Yan QS, Zhang XQ, Xue GP, Huang WG, Wu YF, Zhao YZ, Xue ZY, Huang J, Xu GZ, Wu P (2004) Use of asymmetric somatic hybridization for transfer of the bacterial blight resistance trait from Oryza meyeriana L. to O. sativa L. ssp.japonica. Plant Cell Rep 22:569–575. doi:10.1007/s00299-003-0732-4

    Article  PubMed  CAS  Google Scholar 

  • Yue W, Xia GM, Zhi DY, Chen HM (2001) Transfer of salt tolerance from Aeleuropus littorulis sinensis to wheat (Triticum aestivum L.) via asymmetric somatic hybridization. Plant Sci 161:259–266. doi:10.1016/S0168-9452(01)00382-X

    Article  CAS  Google Scholar 

  • Zhou AF, Chen XL, Xia GM, Chen HM (2002) Study of UV-fusion between common wheat and Haynaldia villosa. Acta Phytophysiol Sinica 28(4):305–310

    CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by grants from National Natural Science Foundation of China (30400287), Guangdong Natural Science Foundation of China (011126, 06023159), Programs for Science and Technology Development of Guangdong (2006B20101014) and Programs for Science and Technology Development of Guangdong (2006Z3-E0281).

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Authors and Affiliations

  1. The Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Zhongshan (Sun Yat-Sen) University, 510275, Guangzhou, Guangdong, China

    Wang Xiao, Xia Huang, Qing Gong, Xue-Mei Dai, Jie-Tang Zhao, Yue-Rong Wei & Xue-Lin Huang

  2. Department of Biology, Guangdong Education Institute, 510303, Guangzhou, Guangdong, China

    Wang Xiao

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  1. Wang Xiao
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  2. Xia Huang
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  3. Qing Gong
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  4. Xue-Mei Dai
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Correspondence to Xue-Lin Huang.

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Xiao, W., Huang, X., Gong, Q. et al. Somatic hybrids obtained by asymmetric protoplast fusion between Musa Silk cv. Guoshanxiang (AAB) and Musa acuminata cv. Mas (AA). Plant Cell Tiss Organ Cult 97, 313–321 (2009). https://doi.org/10.1007/s11240-009-9530-1

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