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Desiccation and cryopreservation of actively-growing cultured plant cells and protoplasts

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Abstract

Actively-growing cultured cells of Pogonatum and Polytrichum were desiccated and cryopreserved. Although Pogonatum was slightly more tolerant to desiccation, both species were cryopreserved with >90% survival rate. An examination of isolated protoplasts revealed that differences in desiccation tolerance were likely dependent on levels of injury of plasma membranes. Trehalose and sucrose provided some protective effects during protoplast desiccation, but mannitol and glucose were less effective when Pogonatum protoplasts were directly desiccated and preserved at various temperatures. The effectiveness of glucose was enhanced when combined with culture medium components.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

ABA:

Abscisic acid

BA:

6-Benzyl aminopurine

MS:

Murashige and Skoog (1962) medium

CI:

Callus induction medium (Takio et al. 1986)

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Acknowledgements

This work was supported partly by the Research Institute for Technology of Tokyo Denki University under Grant Q05E-08 and the Frontier Research and Development Center of Tokyo Denki University under Grant 07FZ03.

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Authors and Affiliations

  1. Graduate School of Advanced Science and Technology, Tokyo Denki University, Hatoyama, Saitama-ken, 350-0039, Japan

    Hideyuki Yamazaki, Katsunori Ayabe, Ryo Ishii & Akira Kuriyama

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  1. Hideyuki Yamazaki
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  2. Katsunori Ayabe
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  3. Ryo Ishii
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  4. Akira Kuriyama
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Correspondence to Akira Kuriyama.

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Yamazaki, H., Ayabe, K., Ishii, R. et al. Desiccation and cryopreservation of actively-growing cultured plant cells and protoplasts. Plant Cell Tiss Organ Cult 97, 151–158 (2009). https://doi.org/10.1007/s11240-009-9509-y

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