Summary
A cryopreservation process using encapsulation/dehydration was set up for apices sampled on in vitro plantlets of sugarcane. After dissection, apices were cultured for one day on standard medium and then encapsulated in medium with 3% alginate. Optimal conditions comprised preculture for 2 days in liquid medium with 250 g.l−1 sucrose, desiccation for 6 hours under the laminar flow or for 10–11 hours with silicagel followed by rapid freezing and slow thawing. Survival after freezing in liquid nitrogen ranged between 38 and 91% for the 5 varieties experimented. Cryopreservation did not modify the electrophoretic profiles for aminoleucine peptidases and amylases with plants of the variety Co 6415.
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Abbreviations
- BAP:
-
6-benzylaminopurine
- KIN:
-
Kinetin
- EDTA:
-
ethylenediamine tetracetic acid
- AMP:
-
aminoleucine peptidases
- AMY:
-
amylases
- RFLP:
-
restriction fragment length polymorphism
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Communicated by A. M. Boudet
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Paulet, F., Engelmann, F. & Glaszmann, JC. Cryopreservation of apices of in vitro plantlets of sugarcane (Saccharum sp. hybrids) using encapsulation/dehydration. Plant Cell Reports 12, 525–529 (1993). https://doi.org/10.1007/BF00236101
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DOI: https://doi.org/10.1007/BF00236101