Abstract
The effects of genotype, pollen or growth regulator-pretreatment of pistils, developmental stage of the ovule (embryo sac) and culture media on induction of gynogenesis, and subsequent plantlet regeneration in vitro were assessed in interspecific Gossypium barbadense × G. hirsutum cotton hybrids. Gynogenesis occurred in all genotypes used when the pistils had been pre-treated with pollen from Hibiscus cannabinus and ovaries were harvested 5 or 10 days after anthesis. The use of culture media, SH and MS, showed no significant differences in responding ovules, embryogenic ovules or embryo germination frequency. Recovered progeny were characterized cytogenetically and microscopically to help documenting their reproductive basis. Root tip chromosome counts of 17 plants established from ovule culture revealed that chromosome numbers ranged from 27 to 44. Although the reproductive mechanisms need to be characterized more extensively by cytological and molecular means, the observations suggest that gynogenesis in cotton involves some unusual reproductive events. Aneuploids could be useful for functional genomic characterization of genome shock, deletion mapping, and germplasm introgression.
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Barrow JR, Katterman F, Williams D (1978) Haploid and diploid callus from anther culture. Crop Sci 18:619–622
Bohanec B, Javornik B, Bohanec B, Kreft I (1994) Induction of gynogenesis in agricultural crops: a review. In: Bohanec B, Javornik B (eds) Proceedings on international colloquium plant biotechnology on agriculture. Ljubljana, Slovenia, pp 43–55
Brazauskas G, Pasakinskiene I, Jahoor A (2004) AFLP analysis indicates no introgression of maize DNA in wheat × maize crosses. Plant Breed 123:117–121. doi:10.1046/j.1439-0523.2003.00927.x
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158. doi:10.1016/0014-4827(68)90403-5
Gao W, Chen ZJ, Yu JZ, Raska D, Kohel RJ, Womack JE, Stelly DM (2004) Wide-cross whole-genome radiation hybrid mapping of cotton (Gossypium hirsutum L.). Genetics 167:1317–1329. doi:10.1534/genetics.103.020479
Hoseman D, Bossoutrot D (1983) Induction of haploid plants from in vitro culture of unpollinated beet (Beta vulgaris L.). Z Pflanzenzuchtg 91:74–77
Kantartzi SK, Roupakias DG (2008a) Breeding barriers between Gossypium spp. and species of the Malvaceae family. J Bot 56:241–245
Kantartzi SK, Roupakias DG (2008b) Production of aneuploids of the cotton hybrid G. barbadense × G. hirsutum L. via intergeneric pollination with Abelmoschus esculentus. Euphytica 161:319–327. doi:10.1007/s10681-007-9550-z
Kumlehn J, Schieder O, Lörz H (1997) In vitro development of wheat (Triticum aestivum L.) from zygote to plant via ovule culture. Plant Cell Rep 16:663–667. doi:10.1007/s002990050298
Lacape JM, Nguyen TB, Courtois B, Belot JL, Giband M, Gourlot JP, Gawryziak G, Roques S, Hau B (2005) QTL analysis of cotton fiber quality using multiple Gossypium hirsutum × Gossypium barbadense backcross generations. Crop Sci 45:123–140
Laurie DA, Bennett MD (1989) The timing of chromosome elimination in hexaploid wheat × maize crosses. Genome 32:953–961
Lux H, Herrman L, Wetzel C (1990) Production of haploid sugar beet (Beta vulgaris L.) by culturing unpollinated ovules. Plant Breed 104:177–183. doi:10.1111/j.1439-0523.1990.tb00420.x
Mavromatis AG, Kantartzi SK, Vlachostergios DN, Xynias IN, Skarakis GN, Roupakias DG (2005) Induction of embryo development and fixation of partial interspecific lines after pollination of F1 cotton interspecific hybrids (Gossypium barbadense × Gossypium hirsutum) with pollen from Hibiscus cannabinus. Aust J Agric Res 56:1101–1109. doi:10.1071/AR04241
Mukhambetzhanov SK (1997) Culture of nonfertilized female gametophytes in vitro. Plant Cell Tissue Organ Cult 48:111–119. doi:10.1023/A:1005838113788
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497. doi:10.1111/j.1399-3054.1962.tb08052.x
Saha S, Wu J, Jenkins JN, McCarty JC, Gutierrez OA Jr, Stelly DM, Percy RG, Raska DA (2004) Effect of chromosome substitutions from Gossypium barbadense L. 3–79 into G. hirsutum L. TM-1 on agronomic and fiber traits. J Cotton Sci 8:162–169
San Noeum LH, Gelebart P (1986) Production of gynogenic haploids. In: Vasil IK (ed) Cell culture and somatic cell genetics of plants, vol 3. Academic Press, New York, pp 305–322
Sibi ML, Kobbaissi A, Shekafandeh A (2001) Green haploid plants from unpollinated ovary culture in tetraploid wheat (Triticum durum Defs.). Euphytica 122:351–359. doi:10.1023/A:1012991325228
Stewart JMD, Hsu CL (1978) Hybridization of diploid and tetraploid cottons through in ovule embryo culture. J Hered 69:404–408
Truong-Andre I, Demarly (1984) Obtaining plants by in vitro culture of unfertilized maize ovaries (Zea mays L.) and preliminary studies on the progeny of gynogenetic plant. Z Pflanzenzuchtg 92:309–320
Wise RR, Sassenrath-Cole GF, Percy R (2000) A comparison of leaf anatomy in field-grown Gossypium hirsutum and G. barbadense. Ann Bot (Lond) 86:731–738. doi:10.1006/anbo.2000.1235
Wu BJ, Chen KC (1982) Cytological and embryological studies on haploid plant production from cultured unpollinated ovaries of Nicotiana tabacum L. Acta Bot Sin 24:125–129
Zhou C, Yang HY (1981) Induction of haploid rice plantlets by ovary culture. Plant Sci Lett 20:231–237. doi:10.1016/0304-4211(81)90267-4
Zhou SQ, Qian DQ, Cao XY (1991) Induction of parthenogenesis and chromosome behavior in plants of parthenogenetic origin in cotton (Gossypium hirsutum). Genome 34:255–260
Znamenskaya VV, Podvigina OA, Zhuzhzhalova TP (1994) Production of new breeding material. Sakharnaya Svekla 6:8
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Kantartzi, S.K., Roupakias, D.G. In vitro gynogenesis in cotton (Gossypium sp.). Plant Cell Tiss Organ Cult 96, 53–57 (2009). https://doi.org/10.1007/s11240-008-9459-9
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DOI: https://doi.org/10.1007/s11240-008-9459-9