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Prospects of androgenetic induction in Lupinus spp.

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Abstract

Anthers cultures of six Polish cultivars of pasture lupin (Lupinus L.) were examined for their androgenic response. Anthers with microspores at the uninucleate stage were isolated from flower buds and cultured in liquid media. Better viability of androgenetic structures was obtained when donor plants had grown under field as opposed to greenhouse conditions. A density of five anthers per 0.5 ml medium was more conducive to androgenetic induction than 25 anthers per 0.5 ml medium. Addition of 5% maltose to the induction medium and culture at 25°C without pre-treatment of flowers, buds or anthers promoted microspore release and division. The greatest frequency of androgenic callus, ~70% was developed from cvs. Katon, Wat (white lupin), in contrast to cvs. Legat, Juno (yellow lupin), Polonez and Sonet (narrow-leafed lupin) with callus induction ~30–40%. Despite various combinations of media tested, plant regeneration was not obtained from anther derived callus.

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Acknowledgement

This study was supported by COST 851 23/E –189/SPB/COST/P-06/DZ 585/2002-2005.

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Authors and Affiliations

  1. Polish Academy of Sciences, The F. Górski Institute of Plant Physiology, Niezapominajek 21, 30-239, Krakow, Poland

    Edyta Skrzypek, Ilona Czyczyło-Mysza, Izabela Marcińska & Maria Wędzony

  2. Pedagogical University of Cracow, Podchorążych 2, 30-084, Krakow, Poland

    Maria Wędzony

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  1. Edyta Skrzypek
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  2. Ilona Czyczyło-Mysza
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  3. Izabela Marcińska
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  4. Maria Wędzony
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Correspondence to Maria Wędzony.

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Skrzypek, E., Czyczyło-Mysza, I., Marcińska, I. et al. Prospects of androgenetic induction in Lupinus spp.. Plant Cell Tiss Organ Cult 94, 131–137 (2008). https://doi.org/10.1007/s11240-008-9396-7

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  • DOI: https://doi.org/10.1007/s11240-008-9396-7

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