Abstract
Shoot cultures of Gentianella austriaca (A. & J. Kerner) Dostal established from seedling epicotyls were maintained on MS medium supplemented with 2.22 μM BA and 0.54 μM NAA. A characteristic feature of these cultures was precocious flowering, which appeared in all rapidly elongating shoots. Flower development arrested shoot elongation and multiplication of shoot cultures. Continuous shoot propagation was possible only by use of small axillary or adventitious buds as explants for subculturing. Flowering could not be suppressed by GA3 addition or by cultivation in short-day conditions. The highest rooting percentage (47.3% with 7.83 roots per explant) was achieved on media with 4.92 μM IBA. Shoot cultures contained the same types of secondary metabolites as plants from nature. Xanthones were the major constituents, with DMB (demethylbellidifolin), DGL (demethylbellidifolin-8-O-glucoside) and BGL (bellidifolin-8-O-glucoside) present at roughly two times lower concentrations than in samples from nature. Secondary metabolite production was strongly affected by the presence of BA in the medium.
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Abbreviations
- BA:
-
Benzyladenine
- BGL:
-
Bellidifolin-8-O-glucoside
- DGL:
-
Demethylbellidifolin-8-O-glucoside
- DMB:
-
Demethylbellidifolin
- GA3 :
-
Gibberellic acid
- IBA:
-
Indole-3-butyric acid
- MS:
-
Murashige and Skoog
- NAA:
-
a-Naphthaleneacetic acid
- WPM:
-
Woody plant medium
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Acknowledgments
We thank Prof Mirjana Nešković for critical reading of the manuscript. The work was sponsored by the Serbian Ministry of Science and Environmental Protection (Grant No. 143026B).
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Vinterhalter, B., Janković, T., Šavikin, K. et al. Propagation and xanthone content of Gentianella austriaca shoot cultures. Plant Cell Tiss Organ Cult 94, 329–335 (2008). https://doi.org/10.1007/s11240-008-9374-0
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DOI: https://doi.org/10.1007/s11240-008-9374-0