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Chromosomes are eliminated in the symmetric fusion between Arabidopsis thaliana L. and Bupleurum scorzonerifolium Willd.

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Abstract

In order to investigate chromosome elimination in symmetric somatic hybridization between Bupleurum scorzonerifolium and Arabidopsis thaliana, protoplasts were isolated from suspension cultures of both A. thaliana and B. scorzonerifolium parents. Biparental protoplasts were mixed at a rate of 1.5:1 and fused with PEG-method. After protoplast fusion, the products were cultured in the P5 liquid medium for microcallus formation. Single cell lines formed from microcalli after subculturing on the MB1 (Xia and Chen, Plant Sci 120:197–203, 1996) solid medium. The putative somatic hybrid cell lines were identified by cytological and molecular analysis. Of the 132 somatic cell lines generated, 16 were identified as somatic hybrids, with the phenotypes resembled B. scorzonerifolium parent. These hybrids showed a complete set of B. scorzonerifolium chromosome and 0–2 small chromosome(s) of A. thaliana. A few of them showed nuclear and cytoplasmic SSR fragments of A. thaliana. These hybrid cell lines could differentiate to green spots, buds/leaves through complementation of regeneration ability. The chromosomes elimination of A. thaliana was discussed.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxi-acetic acid

GISH:

Genomic in situ Hybridization

CTAB:

Cetyl trimethyl ammonium bromide

SSR:

Simple sequence repeats

PEG:

Polyethylene glycol

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Acknowledgements

This work was supported by the National Basic Research Program of China Project No.2006CB100100; National 863 High Technology Research and Development Project No.2006011001020 and Middle-Young’s Scientist Encouraging Foundation by Shandong Science and Technology Committee, Project No. 2005BS03023.

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Authors and Affiliations

  1. School of Life Sciences, Shandong University, Shan Da Nan Lu 27, Jinan, 250100, Shandong, China

    Wang Minqin, Zhao Junsheng, Peng Zhenying, Guo Wei, Wang Yun, Wang Le & Xia Guangmin

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  1. Wang Minqin
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  2. Zhao Junsheng
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  3. Peng Zhenying
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  4. Guo Wei
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  5. Wang Yun
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  6. Wang Le
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  7. Xia Guangmin
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Correspondence to Xia Guangmin.

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Wang Minqin and Zhao Junsheng contributed equally to the work.

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Minqin, W., Junsheng, Z., Zhenying, P. et al. Chromosomes are eliminated in the symmetric fusion between Arabidopsis thaliana L. and Bupleurum scorzonerifolium Willd.. Plant Cell Tiss Organ Cult 92, 121–130 (2008). https://doi.org/10.1007/s11240-007-9308-2

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  • DOI: https://doi.org/10.1007/s11240-007-9308-2

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