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Transgenic Agrostis mongolica Roshev. with enhanced tolerance to drought and heat stresses obtained from Agrobacterium-mediated transformation

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Abstract

Efficient procedures for regeneration and Agrobacterium-mediated transformation were established for Agrostis mongolica Roshev. and generated transgenic plants tolerant to drought and heat stresses using a regulatory gene from Arabidopsis, ABF3, which controls the ABA-dependent adaptive responses. The identification and selection of regenerable and reproducible callus type was a key factor for successful transformation. The transformation efficiency was 49.2% and gfp expression was detected in hygromycin-resistant calli and stem of putative transgenic plants. The result of Southern blot analysis showed that the ABF3 transgene was stably integrated into the genome of transgenic plants. Of the five transgenic lines analyzed, single transgene integration was observed in two lines and two copy integration was observed in three transgenic lines. Northern blot analysis confirmed that ubi::ABF3 was expressed in all transgenic lines. Transgenic plants exhibited neither growth inhibition nor visible vegetative phenotypic alternations. However, both transgenic and wild-type plants were highly sterile and did not flower during 3 years of growth period in the open field under subtropical Jeju Island climate. The stomata of the transgenic plants opened less than did stomata of the wild-type plants, and water content of the transgenic leaves remained about 3–4 fold higher than observed for wild-type leaves under drought stress. The transgenic plants showed about 2 fold higher survival rates under drought stress and about 3 fold higher survival rates under heat stress when compared to wild-type plants. Thus, overexpression of the Arabidopsis ABF3 gene results in enhancement of both drought and heat stress tolerance in Agrostis mongolica Roshev.

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Abbreviations

ABA:

Abscisic acid

ABF:

Abscisic acid responsive element binding factor

ABRE:

Abscisic acid responsive element

bZIP:

Basic region-leucine zipper

NAA:

α-Naphthaleneacetic acid

TDZ:

Thidiazuron

2,4-D:

2,4-Dichlorophenoxyacetic acid

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Acknowledgments

This work was supported by grants from (554–556) Education program for environment favourable agriculture & subtropical bio-industry and the Ministry of Agriculture and Forestry of Korea (Biogreen 21).

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Authors and Affiliations

  1. College of Applied Life Sciences, Cheju National University, Jeju, 690-756, Korea

    Enkhchimeg Vanjildorj, Tae-Woong Bae, Key-Zung Riu & Hyo-Yeon Lee

  2. Jeju Hi-Tech Industry Development Institute, Jeju, 690-121, Korea

    Pil-Yong Yun

  3. Department of Clinical Pathology, Halla College, Jeju, 690-708, Korea

    Shin-Young Park

  4. College of Natural Sciences, Pusan National University, Busan, 609-735, Korea

    Choon-Hwan Lee

  5. Kumho Life and Environmental Science Laboratory, Kwangju, 500-712, Korea

    Soo-Young Kim

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  1. Enkhchimeg Vanjildorj
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  2. Tae-Woong Bae
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  3. Key-Zung Riu
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  6. Choon-Hwan Lee
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  8. Hyo-Yeon Lee
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Correspondence to Hyo-Yeon Lee.

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Vanjildorj, E., Bae, TW., Riu, KZ. et al. Transgenic Agrostis mongolica Roshev. with enhanced tolerance to drought and heat stresses obtained from Agrobacterium-mediated transformation. Plant Cell Tiss Organ Cult 87, 109–120 (2006). https://doi.org/10.1007/s11240-006-9143-x

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  • DOI: https://doi.org/10.1007/s11240-006-9143-x

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