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Agrobacterium-mediated transient transformation of marigold (Tagetes erecta)

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Abstract

Hypocotyls, roots, leaf sections and shoot tips from Tagetes erecta plantlets were inoculated with Agrobacterium tumefaciens, harboring the binary vector pCAMBIA2301, containing the β-glucuronidase gene. Histochemical GUS assays of infected tissues showed transient gus gene expression after 3 days.

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Abbreviations

BA:

6-benzyladenine

GUS:

β-glucuronidase

IAA:

indole-3-acetic acid

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Acknowledgements

This study was supported by the Consejo Nacional de Ciencia y Tecnología (CONACYT) from México (Grant No. 28643-B).

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Authors and Affiliations

  1. Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43 # 130, Chuburná de Hidalgo, Mérida, Yucatán, 97200, México

    Gregorio Godoy-Hernández, Elide Avilés Berzunza, Lizbeth Castro Concha & María de Lourdes Miranda-Ham

Authors
  1. Gregorio Godoy-Hernández
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  2. Elide Avilés Berzunza
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  3. Lizbeth Castro Concha
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  4. María de Lourdes Miranda-Ham
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Correspondence to Gregorio Godoy-Hernández.

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Godoy-Hernández, G., Berzunza, E.A., Concha, L.C. et al. Agrobacterium-mediated transient transformation of marigold (Tagetes erecta). Plant Cell Tiss Organ Cult 84, 365–368 (2006). https://doi.org/10.1007/s11240-005-9031-9

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  • DOI: https://doi.org/10.1007/s11240-005-9031-9

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