Abstract
In vitro plant regeneration of Agave fourcroydes Lem. (Agavaceae) is described. Results suggest that the NO3 -:NH4 + balance in the culture medium is a key factor controlling callus growth and organogenesis in rhizome cultures. Stem callus showed limited organogenic capacity, but high cytokinin concentrations induced adventitious shoot formation on stem explants. When these shoots were excised and subcultured, new callus formed at their base from which new shoots arose. The shoots from stem explants and rhizome callus formed extensive root systems in vitro and were transferred to pot culture with a 90% survival rate.
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Robert, M.L., Herrera, J.L., Contreras, F. et al. In vitro propagation of Agave fourcroydes Lem. (Henequen). Plant Cell Tiss Organ Cult 8, 37–48 (1987). https://doi.org/10.1007/BF00040731
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DOI: https://doi.org/10.1007/BF00040731