Abstract
The in vitro response of kiwifruit (Actinidia deliciosa) to increasing concentrations of boron (B) and NaCl in the culture medium was studied. Kiwifruit shoot cultures were grown in vitro for 12 weeks on an MS medium containing two B concentrations (0.1 and 2 mM) combined with five NaCl concentrations (0, 10, 20, 40 and 80 mM). Kiwifruit produced the longest shoots with 2 mM B when NaCl concentration was 0--20 mM. More shoots were produced with 2 mM B for all NaCl treatments. More shoots were produced with 2 mM B and 10 and 20 mM NaCl. High B concentrations in the culture medium significantly increased shoot proliferation. Explants exhibited a moderate chlorotic appearance with 40 mM NaCl and shoots died with 80 mM NaCl. With 2 mM B, the B concentration of explants was 5--9X greater for the various NaCl treatments compared to the control. Increasing the NaCl concentration from 10 to 80 mM, resulted in higher Na and Cl concentrations in explants for all B treatments, while K and Ca concentrations decreased. Phosphorus concentration in the explants was significantly increased by increasing the NaCl concentration reaching a maximum value at 80 mM NaCl for the two B concentrations.
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References
Dimassi-Theriou K (1998) Response of increasing rates of NaCl or CaCl2 and proline on ‘Mr.S 2/5’ (Prunus cerasifera) peach rootstock cultured in vitro. Adv. Hort. Sci. 12: 169–174
Eshel A (1985) Response of Suaeda aegyptiaca to KCl, NaCl and Na2SO4 treatments. Plant Physiol. 64: 308–315
Flowers TJ & Lauchli A (1983) Sodium versus potassium: substitution and compartmentation. In: Pirson A & Zimmerman MH (eds) Encyclopedia of Plant Physiology New Series, Vol. 15 (pp. 651–681). Springer-Verlag, Berlin
Gupta UC (1983) Boron deficiency and toxicity symptoms for several crops as related to tissue boron levels. J. Plant Nutr. 6: 387–395
Heysher JW & Nabors MW (1981) Osmotic adjustment of cultured tobacco cells (Nicotiana tabacum var. Samsum) grown on sodium chloride. Plant Physiol. 67: 720–727
Hu H, Brown PH & Labavitch MJ (1996) Species variability in boron requirement is correlated with cell wall pectin. J. Exp. Bot. 47: 227–232
Kingsbury RW, Epstein E & Pearcy RW (1984) Physiological responses to salinity in selected lines of wheat. Plant Physiol. 74: 417–423
Kwon T, Abe T & Sasahara T (1995) Enhanced saline stress resistance in threonine and methionine overproducing mutant cell line from protoplast culture of rice (Oryza sativa L.). J. Plant Physiol. 145: 551–556
Lehle FR, Chen F & Wendt KR (1992). Enhancement of NaCl tolerance in Arabidopsis thaliana by exogenous L-asparagine and D-asparagine. Physiol. Plant 84: 223–228
Loomis WD & Durst RW (1992) Chemistry and biology of boron. Biofactors 3: 229–239
Lutts S, Kinet JM & Bouharmont J (1999) Improvement of rice callus regeneration in the presence of NaCl. Plant Cell Tiss. Org. Cult. 57: 3–11
Maas EV & Hoffman GJ (1977) Crop salt tolerance: evaluation of existing data. In: Drogne HE (ed) Managing Saline Water for Irrigation (pp. 197–198). Texas Tech. Univ. Press, Lubbock
Matter J & Turian G (1961) Action antimitotique de l'acide borique dans le meristeme radiculaire de graines de Lactuca sativa en germination. Bull. Soc. Botan. Suisse. 71: 361–369
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Plant Physiol. 15: 473–497
Nable RO, Banuelos GS & Paull JG (1997) Boron toxicity. Plant Soil. 198: 181–198
O’ Neill MA, Eberhard S, Albersheim P & Darvill AG (2001) Requirement of borate cross-linking of cell wall rhamnogalacturonan II for Arabidopsis growth. Science 294: 846–849
Paek KY, Chandler SF & Thorpe TA (1988) Physiological effects of Na2SO4 and NaCl on callus cultures of Brassica campestris (Chinese cabbage). Physiol. Plant 72: 160–166
Richards LA (1954) Diagnosis and improvement of saline and alkali soils. Agr. Hand. (p. 160) US Dept. Agric. No 60
Robertson GA & Loughman BC (1974) Response to boron deficiency: a comparison with responses produced by chemical methods of retarding root elongation. New Phytol. 73: 821–832
Seresinhe PSJW & Oertli JJ (1991) Effects of boron on growth of tomato cell suspensions. Physiol. Plant 81: 31–36
Silva JAB, Otoni WC, Martinez CA, Dias LM & Silva MAP (2001) Microtuberization of Andean potato species (Solanum spp.) as affected by salinity. Sci. Hort. 89: 91–101
Sotiropoulos TE, Therios IN & Dimassi KN (1998a) Seasonal variation and distribution of soil and plant boron concentrations of kiwifruit orchards irrigated with high boron water. Agrochimica XLII (6): 284–295
Sotiropoulos TE, Therios IN & Dimassi KN (1998b) The effect of toxic boron concentrations on shoot proliferation of in vitro kiwifruit shoot tip cultures. Adv. Hort. Sci. 12: 196–200
Stavarek SJ & Rains DW (1984) The development of tolerance to mineral stress. HortScience 19: 13–18
Wolf B (1974) Improvement in the azomethine-H method for the determination of boron. Comm. Soil Sci. Plant Anal. 5: 39–44
Yamaguchi T, Hara T & Sonoda Y (1986) Distribution of calcium and boron in the pectin fraction of tomato leaf cell wall. Plant Cell Physiol. 27: 729–732
Zhang Y, Abdulnour JE, Donelly DJ & Barthakur NN (2001) Effects of NaCl stress on yield of potato plants derived from previously saline conditions. HortScience 36: 770–771
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Sotiropoulos, T.E., Dimassi, K.N. Response to increasing rates of boron and NaCl on shoot proliferation and chemical composition of in vitro kiwifruit shoot cultures. Plant Cell, Tissue and Organ Culture 79, 285–289 (2004). https://doi.org/10.1007/s11240-004-4609-1
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DOI: https://doi.org/10.1007/s11240-004-4609-1