In vitro propagation of the genus Clivia

Abstract

We have developed a protocol for the in vitro propagation of the genus Clivia. Shoots were regenerated when fragments of the peduncle-pedicel junction (PP junction) from young inflorescences were used as explants. The optimal media for PP junction were Murashige and Skoog (MS)-based medium containing 10 μM of 6-benzyladenine (BA) and 10 μM of 2,4-dichlorophenoxyacetic acid (2,4-D) or MS supplemented with 5 μM BA, 10 μM α-naphthaleneacetic acid (NAA), 250 mg l^-1 glutamine and 500 mg l⁻¹ casein hydrolysate and their usage depended on the breeding lines. Multiplication from initiations and in vitro seedlings was the best when the explants were cut longitudinally through the meristem and placed on MS plus 44 μM BA. Plantlets were transferred on to hormone -free MS medium with charcoal for rooting.