Abstract
Mature embryos and seedlings from mature embryos of one standard and five reconstructed karyotypes of barley (Hordeum vulgare L.) were cultured in vitro to study the influence of repositioning of particular chromosome segments of barley genome on the regeneration response. A comparative analysis of the regeneration response of a reconstructed karyotype having complete and well characterized rearrangement of the chromosome complement, and its four parental lines were used as experimental material. Depending on the source of explants two systems of in vitro culture were applied. The regeneration ability was found to be significantly influenced by both chromosome reconstruction and protocol applied. Possible reasons underlying the effects of chromosomal reconstruction on the regeneration response of karyotypes are briefly discussed.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- IAA:
-
indole-3-acetic acid
- 2ip:
-
6-(γ,γ-dimethylallylamino)purine
References
P Bregitzer R Campbell (2001) ArticleTitleGenetic markers associated with green and albino plant regeneration from embryogenic barley callus Crop Sci. 41 173–179
OL Gamborg RA Miller K Ojima (1968) ArticleTitleNutrient requirements of suspension cultures of soybean root cells Exp. Cell Res. 50 151–158
KI Gecheff (1989) ArticleTitleMultiple reconstruction of the barley karyotypes resulting in complete cytological marking of the chromosome complement Theor. Appl. Genet. 78 683–688
KI Gecheff (1991) ArticleTitleFurther evidence for high position-specific effects in the action of chemical mutagens on chromosomes of barley Theor. Appl. Genet. 83 179–184
JJ Hanzel JP Miller MA Brinkman E Fendos (1985) ArticleTitleGenotype and media effects on callus formation and regeneration in barley Crop Sci. 25 27–31
R Lührs H Lörz (1987) ArticleTitlePlant regeneration in vitro from embryogenic cultures of spring- and winter-type barley, Hordeum vulgare Theor. Appl. Genet. 75 16–25
Y Mano H Takahashi K Sato K Takeda (1996) ArticleTitleMapping genes for callus growth and shoot regeneration in barley Breed. Sci. 46 137–142
Y Mano T Komatsuda (2002) ArticleTitleIdentification of QTLs controlling tissue-culture traits in barley (Hordeum vulgare L.) Theor. Appl. Genet. 105 708–715
T Murashige F Skoog (1962) ArticleTitleA revised medium for rapid growth, bioassays with tobacco tissue cultures Physiol. Plant. 15 473–497
N Papazova T Hvarleva A Atanassov K Gecheff (2001) ArticleTitleThe role of cytosine methylation for rRNA gene expression in reconstructed karyotypes of barley Biotechnol. Biotech. Eq. 15 35–44
K Rikiiski S Yasuda (1994) ArticleTitleComparison of regenerating ability of calli derived from mature and immature embryos in barley varieties Bull. Res. Inst. Bioresour. Okayama Uni 2 33–42
I Schubert G Künzel (1990) ArticleTitlePosition-dependent NOR activity in barley Chromosoma 99 352–359
Z Vitanova I Vitanov A Trifonova D Savova A Atanassov (1995) ArticleTitleEffect of 2,4-D, precultivation on regeneration capacity of cultivated barley Plant Cell Rep. 14 437–441
Y Wan PG Lemaux (1994) ArticleTitleGeneration of large numbers of independently transformed fertile barley plants Plant Physiol. 104 37–48
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Abumhadi, N., Kamenarova, K., Gecheff, K. et al. Effects of chromosome reconstruction of barley genome on tissue culture response using two different regeneration systems. Plant Cell Tiss Organ Cult 80, 339–342 (2005). https://doi.org/10.1007/s11240-004-0912-0
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DOI: https://doi.org/10.1007/s11240-004-0912-0