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DNA interaction with synthetic polymers in solution

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Abstract

DNA complexes with cationic polymers (polyvinylamine (PVA), polyallylamine (PAA), polydimethylaminoethylmethacrylate (PDMAEM), poly-(N,N,N-trimethylammonio)ethyl methacrylate chloride (PTMAEM), poly-l-lysine (PLL)) were investigated. It was shown that volume and persistent length of DNA do not change essentially at low cationic polymer concentration in a solution. DNA packaging in 0.005 M NaCl was observed at charge ratio N/P ≈ 1. Secondary DNA structure in complexes was not disrupted, and DNA was protected from protonation. The comparison between DNA packaging in complexes with polycations and DNA condensation induced by trivalent ions was made.

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Authors and Affiliations

  1. Department of Molecular Biophysics, Faculty of Physics, St. Petersburg State University, Uluanovskaya St., 1, Petrodvorets, St.-Petersburg, 198504, Russia

    Nina Kasyanenko, Daria Afanasieva, Boris Dribinsky & Dmitry Mukhin

  2. Institute of Macromolecular Compounds RAS, St. Petersburg, Russia

    Olga Nazarova & Evgeny Panarin

Authors
  1. Nina Kasyanenko
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  2. Daria Afanasieva
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  3. Boris Dribinsky
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  4. Dmitry Mukhin
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  5. Olga Nazarova
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  6. Evgeny Panarin
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Correspondence to Nina Kasyanenko.

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Kasyanenko, N., Afanasieva, D., Dribinsky, B. et al. DNA interaction with synthetic polymers in solution. Struct Chem 18, 519–525 (2007). https://doi.org/10.1007/s11224-007-9162-1

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  • DOI: https://doi.org/10.1007/s11224-007-9162-1

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