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Activity of the System for Chlorophyll Biosynthesis and Structural and Functional Organization of Chloroplasts in a Plastome en:chlorina-5 Sunflower Mutant

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Abstract

Chlorophyll (Chl) deficiency in leaves of a plastome sunflower (Helianthus annuus L.) en:chlorina-5 mutant is due to the formation of smaller chloroplasts with a markedly reduced membrane system, as compared to the parent 3629 line. Abnormalities in the structure of the photosynthetic apparatus in the mutant can be mainly attributed to changes in the formation of photosystem I and its light-harvesting complexes. Chl deficiency in en:chlorina-5 correlated with its lower capability of synthesizing the first specific Chl precursor, 5-aminolevulinic acid (ALA) in the light. Light-independent stages of Chl biosynthesis in the mutant had the same efficiency as in leaves of the parent line. ALA formation in darkness and its conversion into protochlorophyllide did not depend on the extent of photosynthetic membrane development and photosynthetic activity.

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Abbreviations

ALA:

5-aminolevulinic acid

Chl:

chlorophyll

MgPp(E):

monomethyl ester of MgPp

ETR:

the efficiency of electron transfer chain functioning

LHC:

light-harvesting complex

Pchlide:

protochlorophyllide

POR:

Pchlide-NADPH oxidoreductase

Pp:

protoporphyrin IX

PS:

photosystem

RC:

reaction center

ϕPSII :

effective quantum yield of the primary charge separation in the RCs of PSII

qP:

photochemical and

NPQ:

nonphotochemical quenching of Chl a fluorescence

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Authors and Affiliations

  1. Institute of Biophysics and Cell Engineering, National Academy of Sciences of Belarus, Akademicheskaya ul. 27, Minsk, 220072, Belarus

    V. V. Rassadina & N. G. Averina

  2. Rostov State University, Rostov-on-Don, Russia

    A. V. Usatov & G. M. Fedorenko

Authors
  1. V. V. Rassadina
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  2. A. V. Usatov
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  3. G. M. Fedorenko
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  4. N. G. Averina
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__________

Translated from Fiziologiya Rastenii, Vol. 52, No. 5, 2005, pp. 683–693.

Original Russian Text Copyright © 2005 by Rassadina, Usatov, Fedorenko, Averina.

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Rassadina, V.V., Usatov, A.V., Fedorenko, G.M. et al. Activity of the System for Chlorophyll Biosynthesis and Structural and Functional Organization of Chloroplasts in a Plastome en:chlorina-5 Sunflower Mutant. Russ J Plant Physiol 52, 606–615 (2005). https://doi.org/10.1007/s11183-005-0090-x

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  • DOI: https://doi.org/10.1007/s11183-005-0090-x

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