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A potent antifungal rhizobacteria Bacillus velezensis RB.DS29 isolated from black pepper (Piper nigrum L.)

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Abstract

Five hundred strains of rhizobacteria were isolated from the rhizosphere of the Central Highlands of Vietnam, where black pepper is cultivated. Of these, seven potent rhizobacteria were evaluated for anti-Phytophthora activity and 16S rRNA gene sequencing and phylogenic analysis classified. Evaluation of their antifungal activity was performed both in vitro and in vivo. The results showed that almost all potent rhizobacteria possessed anti-Phytophthora activity. The rhizobacteria strains displayed over 60% inhibition of Phytophthora during the in vitro test, and six rhizobacteria inhibited Phytophthora by 77.50–98.75% during the in vivo test. Enzymatic activities were measured to determine the antifungal mechanisms; these were identified as protease, chitinase, and β-glucanase. The effects of the rhizobacteria on plant growth and antifungal activity were also investigated. Under greenhouse conditions, black pepper seedlings treated with rhizobacteria were stronger and had lower rates of disease and fatality compared to the control group. The results from the in vitro test also showed that the anti-Phytophthora activity of the rhizobacteria was not dependent on enzyme activity, but rather on their chemical compounds. GC–MS and LC–MS profiles of the culture broth from the promising rhizobacteria strain RBDS.29 revealed seven potent antifungal compounds. The data suggest that Bacillus velezensis RB.DS29 is a promising rhizobacterium that promotes plant growth and the biocontrol of black pepper.

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Acknowledgements

Authors are expressed to thank Ministry of Education and Training, Vietnam granted the Science and Technology program: Application of Biotechnology for sustainable black pepper production in the Central highland. B 2017–2019. This work was also supported in part by a grant from the Ministry of Science and Technology, Taiwan (MOST 106-2320-B-032-001-MY3).

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Authors and Affiliations

  1. Department of Science and Technology, Tay Nguyen University, Buon Ma Thuot, 630000, Vietnam

    Thi Huyen Trang Trinh, Van Bon Nguyen, Chien Thang Doan & Thi Phuong Khanh Vo

  2. Department of Chemistry, Tamkang University, New Taipei City, 25137, Taiwan

    San-Lang Wang

  3. Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot, 630000, Vietnam

    Minh Dinh Tran & Anh Dzung Nguyen

  4. Department Plant Pathology, University California, One Shields Avenue, Davis, CA, 95616, USA

    Que V. Huynh

Authors
  1. Thi Huyen Trang Trinh
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  2. San-Lang Wang
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  3. Van Bon Nguyen
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  4. Minh Dinh Tran
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  5. Chien Thang Doan
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  6. Thi Phuong Khanh Vo
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  7. Que V. Huynh
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  8. Anh Dzung Nguyen
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Contributions

ADN conceived the study. ADN, HTT, and VBN designed and performed the study. AND and S-LW contributed the reagents/materials/analysis tools. QVH, CTD, VBN, and PKV analyzed the data. AND and S-LW wrote the paper.

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Correspondence to San-Lang Wang or Anh Dzung Nguyen.

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Trinh, T.H.T., Wang, SL., Nguyen, V.B. et al. A potent antifungal rhizobacteria Bacillus velezensis RB.DS29 isolated from black pepper (Piper nigrum L.). Res Chem Intermed 45, 5309–5323 (2019). https://doi.org/10.1007/s11164-019-03971-5

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  • DOI: https://doi.org/10.1007/s11164-019-03971-5

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