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The isolation of chitinase from Streptomyces thermocarboxydus and its application in the preparation of chitin oligomers

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Abstract

Microbial chitinase has received great attention due to its medical, biological, and agricultural applications. In this study, over 50 bacterial strains were isolated from Taiwanese soils using medium containing squid pen powder (SPP) as the sole source of carbon/nitrogen. Based on chitinolytic activity, Streptomyces thermocarboxydus TKU045 was selected for further study. Optimized culture conditions revealed that S. thermocarboxydus TKU045 could produce the highest chitinase activity (52.985 U/mL) when cultured in a medium containing 1% (w/v) SPP at 45 °C for 36 h. Characterized TKU045 chitinase showed novel properties with a smallest molecular weight (12.8 kDa by sodium dodecyl sulfate–polyacrylamide gel electrophoresis analysis) and more acidic optimal pH (pH 4) than those of other Streptomyces chitinases. A combination of high-performance liquid chromatography and matrix-assisted laser desorption ionization time of flight mass spectrometer data revealed that chitin oligomers (COS) obtained from the hydrolysis of colloidal chitin by TKU045 chitinase comprise oligomers with multiple degrees of polymerization (DP) varying from 1 to 7. The COS with low DP exhibited enhanced 2,2-diphenyl-1-picrylhydrazyl radical scavenging capability and promoted the growth of Lactobacillus lactis. Taken together, the COS obtained by hydrolyzing colloidal chitin with TKU045 chitinase could have the potential to be used in medicine or nutraceuticals due to its active anti-oxidant and prebiotic contents.

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References

  1. S.L. Wang, T.W. Liang, Res. Chem. Interm. 43, 3445 (2017)

    Article  CAS  Google Scholar 

  2. G. Akca, A. Özdemir, Z.G. Öner, S. Şenel, Res. Chem. Interm. 44, 4811 (2018)

    Article  CAS  Google Scholar 

  3. F. Ding, H. Li, Y. Du, X. Shi, Res. Chem. Interm. 44, 4827 (2018)

    Article  CAS  Google Scholar 

  4. M.M. Jaworska, A. Górak, Res. Chem. Interm. 44, 4841 (2018)

    Article  CAS  Google Scholar 

  5. L.C. Tsai, M.L. Tsai, K.Y. Lu, F.L. Mi, Res. Chem. Interm. 44, 4855 (2018)

    Article  CAS  Google Scholar 

  6. A. Mohandas, W. Sun, T.R. Nimal, S.A. Shankarappa, N.S. Hwang, R. Jayakumar, Res. Chem. Interm. 44, 4873 (2018)

    Article  CAS  Google Scholar 

  7. P. Hiranpattanakul, T. Jongjitpissamai, S. Aungwerojanawit, W. Tachaboonyakiat, Res. Chem. Interm. 44, 4913 (2018)

    Article  CAS  Google Scholar 

  8. D. Kotatha, K. Morishima, S. Uchida, M. Ogino, M. Ishikawa, T. Furuike, H. Tamura, Res. Chem. Interm. 44, 4971 (2018)

    Article  CAS  Google Scholar 

  9. T.W. Liang, W.T. Chen, Z.H. Lin, Y.H. Kuo, A.D. Nguyen, P.S. Pan, S.L. Wang, Int. J. Mol. Sci. 17, 1302 (2016)

    Article  CAS  PubMed Central  Google Scholar 

  10. S. Sinha, S. Chand, P. Tripathi, Appl. Biochem. Biotechnol. 180, 883 (2016)

    Article  CAS  PubMed  Google Scholar 

  11. Q. Xiong, Y. Wei, H. Xie, Z. Feng, Y. Gan, C. Wang, M. Liu, F. Bai, F. Xie, G. Shao, Vaccine 32, 3445 (2014)

    Article  CAS  PubMed  Google Scholar 

  12. C. Villiers, M. Chevallet, H. Diemer, R. Couderc, H. Freitas, A. Van Dorsselaer, P.N. Marche, T. Rabilloud, Mol. Cell. Proteomics 8, 1252 (2009)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. D.V. Gerasimenko, I.D. Avdienko, G.E. Bannikova, OYu. Zueva, V.P. Varlamov, Appl. Biochem. Micro. 40, 253 (2004)

    Article  CAS  Google Scholar 

  14. S. Sinha, P. Tripathi, S. Chand, Appl. Biochem. Biotechnol. 167, 1029 (2012)

    Article  CAS  PubMed  Google Scholar 

  15. P.J. Park, J.Y. Je, S.K. Kim, Carbohydr. Polym. 55, 17 (2004)

    Article  CAS  Google Scholar 

  16. W.J. Jung, R.D. Park, Mar Drugs 12, 5328 (2014)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. M. Kuddus, I.Z. Ahmad, J. Gen. Eng. Biotechnol. 11, 39 (2013)

    Article  Google Scholar 

  18. S.L. Wang, T.W. Liang, Y.H. Yen, Carbohydr. Polym. 84, 732 (2011)

    Article  CAS  Google Scholar 

  19. S.L. Wang, H.T. Yu, M.H. Tsai, C.T. Doan, V.B. Nguyen, V.C. Do, A.D. Nguyen, Res. Chem. Interm. 44, 4903 (2018)

    Article  CAS  Google Scholar 

  20. C.L. Wang, J.W. Su, T.W. Liang, A.D. Nguyen, S.L. Wang, Res. Chem. Interm. 40, 2237 (2014)

    Article  CAS  Google Scholar 

  21. T.W. Liang, Y.Y. Chen, P.S. Pan, S.L. Wang, Int. J. Biol. Macromol. 63, 8 (2014)

    Article  CAS  PubMed  Google Scholar 

  22. T.W. Liang, T.Y. Shieh, S.L. Wang, Bioproc. Biosyst. Eng. 37, 1201 (2014)

    Article  CAS  Google Scholar 

  23. S.L. Wang, C.P. Liu, T.W. Liang, Carbohydr. Polym. 90, 1305 (2012)

    Article  CAS  PubMed  Google Scholar 

  24. T.W. Liang, J.L. Hsieh, S.L. Wang, Carbohydr. Res. 362, 38 (2012)

    Article  CAS  PubMed  Google Scholar 

  25. S.L. Wang, P.C. Wu, T.W. Liang, Carbohydr. Res. 344, 979 (2009)

    Article  CAS  PubMed  Google Scholar 

  26. K.J. Kim, Y.J. Yang, J.G. Kim, J. Biochem. Mol. Biol. 36, 185 (2003)

    CAS  PubMed  Google Scholar 

  27. Y. Han, B. Yang, F. Zhang, X. Miao, Z. Li, Mar. Biotechnol. 11, 132 (2009)

    Article  CAS  PubMed  Google Scholar 

  28. G.C. Pradeep, H.Y. Yoo, Y.H. Choi, J.C. Yoo, Appl. Biochem. Biotechnol. 175, 372 (2015)

    Article  CAS  Google Scholar 

  29. M. Rabeeth, A. Anitha, G. Srikanth, Pak. J. Biol. Sci. 14, 788 (2011)

    Article  CAS  PubMed  Google Scholar 

  30. A. Nagpure, P.K. Gupta, J. Basic Microbiol. 53, 429 (2013)

    Article  CAS  PubMed  Google Scholar 

  31. M. Gangwar, V. Singh, A.K. Pandey, C.K. Tripathi, Indian J. Exp. Biol. 54, 64 (2016)

    PubMed  Google Scholar 

  32. C.T. Doan, T.N. Tran, V.B. Nguyen, A.D. Nguyen, S.L. Wang, Mar. Drugs 16, 83 (2018)

    Article  CAS  PubMed Central  Google Scholar 

  33. T.W. Liang, S.C. Tseng, S.L. Wang, Mar. Drugs 14, 40 (2016)

    Article  CAS  PubMed Central  Google Scholar 

  34. S.L. Wang, C.W. Yang, T.W. Liang, C.L. Wang, Carbohydr. Polym. 78, 205 (2009)

    Article  CAS  Google Scholar 

  35. S.L. Wang, C.L. Lin, T.W. Liang, K.C. Liu, Y.H. Kuo, Bioresour. Technol. 100, 316 (2009)

    Article  CAS  PubMed  Google Scholar 

  36. A.D. Nguyen, C.C. Huang, T.W. Liang, V.B. Nguyen, P.S. Pan, S.L. Wang, Carbohydr. Polym. 108, 331 (2014)

    Article  CAS  PubMed  Google Scholar 

  37. N. Shekhar, D. Bhattacharya, D. Kumar, R.L. Gupta, Can. J. Microbiol. 52, 805 (2006)

    Article  CAS  PubMed  Google Scholar 

  38. P. Mander, S.S. Cho, Y.H. Cho, S. Panthi, Y.S. Cho, H.M. Kim, J.C. Yoo, Arch. Pharm. Res. 39, 878 (2016)

    Article  CAS  PubMed  Google Scholar 

  39. N. Karthik, P. Binod, A. Pandey, Bioresour. Technol. 188, 195 (2015)

    Article  CAS  PubMed  Google Scholar 

  40. M.A. Rahman, Y.H. Choi, G.C. Pradeep, J.C. Yoo, Arch. Pharmac. Res. 37, 1522 (2014)

    Article  CAS  Google Scholar 

  41. G.J. Joo, Biotechnol. Lett. 27, 1483 (2005)

    Article  CAS  PubMed  Google Scholar 

  42. G. Mukherjee, S.K. Sen, Curr. Microbiol. 53, 265 (2006)

    Article  CAS  PubMed  Google Scholar 

  43. S.L. Wang, T.Y. Huang, C.Y. Wang, T.W. Liang, Y.H. Yen, Y. Sakata, Bioresour. Technol. 99, 5436 (2008)

    Article  CAS  PubMed  Google Scholar 

  44. S.L. Wang, J.Y. Liou, T.W. Liang, K.C. Liu, Process Biochem. 44, 854 (2009)

    Article  CAS  Google Scholar 

  45. S.L. Wang, D.Y. Kao, C.L. Wang, Y.H. Yen, M.K. Chern, Y.H. Chen, Enzyme Microb. Technol. 39, 724 (2006)

    Article  CAS  Google Scholar 

  46. V.B. Nguyen, A.D. Nguyen, S.L. Wang, Mar. Drugs 15, 274 (2017)

    Article  CAS  PubMed Central  Google Scholar 

  47. S.L. Wang, H.T. Li, L.J. Zhang, Z.H. Lin, Y.H. Kuo, Mar. Drugs 14, 183 (2016)

    Article  CAS  PubMed Central  Google Scholar 

  48. T.W. Liang, S.C. Tseng, S.L. Wang, Mar. Drugs 14, 40 (2016)

    Article  CAS  PubMed Central  Google Scholar 

  49. T.W. Liang, C.C. Wu, W.T. Cheng, Y.C. Chen, C.L. Wang, I.L. Wang, S.L. Wang, Appl. Biochem. Biotechnol. 172, 933 (2014)

    Article  CAS  PubMed  Google Scholar 

  50. C.L. Wang, T.H. Huang, T.W. Liang, S.L. Wang, New Biotechnol. 28, 559 (2011)

    Article  CAS  Google Scholar 

  51. S.L. Wang, C.Y. Wang, Y.H. Yen, T.W. Liang, S.Y. Chen, C.H. Chen, Process Biochem. 47, 1684 (2012)

    Article  CAS  Google Scholar 

  52. S.L. Wang, W.H. Hsu, T.W. Liang, Carbohydr. Res. 345, 880 (2010)

    Article  CAS  PubMed  Google Scholar 

  53. S.L. Wang, W.T. Chang, Appl. Environ. Microbiol. 63, 380 (1997)

    CAS  PubMed  PubMed Central  Google Scholar 

  54. T.W. Liang, Y.J. Chen, Y.H. Yen, S.L. Wang, Process Biochem. 42, 527 (2007)

    Article  CAS  Google Scholar 

  55. K. Kim, N. Rajapakse, Carbohydr. Polym. 62, 357 (2005)

    Article  CAS  Google Scholar 

  56. H.W. Lee, Y.S. Parkb, J.S. Jungb, W.S. Shinb, Anaerobe 8, 319 (2002)

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgment

This work was supported in part by a grant from the Ministry of Science and Technology, Taiwan (MOST 106-2320-B-032-001-MY3).

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Authors and Affiliations

  1. Department of Chemistry, Tamkang University, New Taipei City, 25137, Taiwan

    Thi Ngoc Tran, Chien Thang Doan & San-Lang Wang

  2. Department of Science and Technology, Tay Nguyen University, Buon Ma Thuot, 630000, Vietnam

    Thi Ngoc Tran, Chien Thang Doan & Van Bon Nguyen

  3. Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot, 630000, Vietnam

    Anh Dzung Nguyen

  4. Life Science Development Center, Tamkang University, New Taipei City, 25137, Taiwan

    San-Lang Wang

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  1. Thi Ngoc Tran
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  2. Chien Thang Doan
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Contributions

Conceived the study: S-LW. Designed and performed the study: S-LW, TNT. Contributed reagents/materials/analysis tools: S-LW. Analyzed data: TNT, CTD, VBN, ADN. Wrote the paper: S-LW, TNT.

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Correspondence to San-Lang Wang.

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Tran, T.N., Doan, C.T., Nguyen, V.B. et al. The isolation of chitinase from Streptomyces thermocarboxydus and its application in the preparation of chitin oligomers. Res Chem Intermed 45, 727–742 (2019). https://doi.org/10.1007/s11164-018-3639-y

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