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Reclamation of rhizobacteria newly isolated from black pepper plant roots as potential biocontrol agents of root-knot nematodes

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Abstract

Rhizobacteria have been of great interest due to their promising use in biocontrol and promotion of plant growth. The aim of this study was to select active anti-nematode rhizobacteria, enhance their nematicidal activity by optimizing fermentation conditions, and then determine the active components of the fermented product. Among more than 100 isolates, 20 strains showed potent inhibition (81.67–99%) against root-knot nematodes from the genus Meiloidogyne. Almost all of these belong to the genus Bacillus (14 of 20 strains). The six most active strains (with max inhibition of 96.13–98.33%) were RB.DS.05, RB.DL.28, RB.DS.29, RB.DL.31, H11, and CC01; these strains were further investigated for their effect on nematode egg hatching. Of these, Bacillus subtilis RB.DL.28 demonstrated the greatest inhibition (82%). Chitinase and protease were closely associated with the inhibition of egg hatching, while it was newly discovered that natural compounds with thermal stability play a key role in killing J2 nematodes. Based on LC–MS analysis, two major constituents, sulfacetamide and metronidazole-OH, were identified from the filtrates of B. subtilis RB.DL.28. The results from this study suggest that the most active strain, B. subtilis RB.DL.28, and other newly identified rhizobacteria may be good candidates for biocontrol of Meiloidogyne spp. These results expand the catalogue of novel active anti-nematode rhizobacteria communities recently recorded in Vietnam.

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Acknowledgements

This work was supported in part by a grant from Ministry of Education and Training, Vietnam: Biotechnology application for sustainable black pepper production in the Central Highland (B 2017-2019), and a grant from the Ministry of Science and Technology, Taiwan (MOST 106-2320-B-032-001-MY3).

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Authors and Affiliations

  1. Institute of Research and Development, Duy Tan University, Da Nang, 550000, Vietnam

    Van Bon Nguyen

  2. Department of Chemistry, Tamkang University, New Taipei City, 25137, Taiwan

    San-Lang Wang

  3. Life Science Development Center, Tamkang University, New Taipei City, 25137, Taiwan

    San-Lang Wang

  4. Department of Science and Technology, Tay Nguyen University, Buon Ma Thuot, 630000, Vietnam

    Thi Hanh Nguyen, Thi Huyen Trang Trinh & Thi Thiep Nong

  5. Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot, 630000, Vietnam

    Thi Huyen Nguyen, To Uyen Nguyen & Anh Dzung Nguyen

  6. Faculty of Agriculture and Forestry, Tay Nguyen University, Buon Ma Thuot, 630000, Vietnam

    Van Nam Nguyen

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  1. Van Bon Nguyen
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Correspondence to San-Lang Wang, Van Nam Nguyen or Anh Dzung Nguyen.

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Nguyen, V.B., Wang, SL., Nguyen, T.H. et al. Reclamation of rhizobacteria newly isolated from black pepper plant roots as potential biocontrol agents of root-knot nematodes. Res Chem Intermed 45, 5293–5307 (2019). https://doi.org/10.1007/s11164-019-03970-6

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