Abstract
The recovery of polyphenols from olive mill wastewaters (OMWs) provides the double opportunity to obtain high-added value biomolecules and to reduce the phytotoxicity of the effluent. The purpose of this study is to gain information about the hygienic quality of the raw material by performing a microbiological analysis of olive mill wastewater, from an extraction system of three phases. The determination of total coliforms,fecal coliforms,aecal streptococci, and another group of microorganisms have been explored, namely yeasts, molds and total aerobic mesophilic flora. We examined the results of the confrontation of olive mill wastewater polyphenols with certain strains known for their pathogenicity, and the minimum concentrations inhibiting bacteria and the minimal bactericidal concentration (MBC) were determined by the dilution method. Polyphenols are particularly the delipidated fraction of OMW extracts according to liquid–liquid phase. The overall yield is 1.367 ± 0.108 %. The quantitative estimation of total polyphenols was assessed by the colorimetric reagent Folin–Ciocalteu, gallic acid and tyrosol, tannic acid and total flavonoids catechin (the method using aluminum trichloride). The results of microbiological analysis confirmed that there is no fecal contamination. For the in vitro bioassay, polyphenols showed a variable antimicrobial activity against six bacteria: Escherichia coli, Proteus sp., Pseudomonas aeruginosa, Enterroccus feculis, Staphylococcus aureus, and Klebsiella pneumoniae. This variability depends essentially on the microorganism tested and the nature of the product tested. Indeed, we found complete inhibition of the growth of Proteus sp. and E. fecalis by the sinks method. Some microorganisms were sensitive to phenols with MBC values between 1/50 and 1/25.
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C.H. Lu, W.N. Chang, Y.C. Chuang, H.W. Chang, The prognostic factors of adult gram-negative bacillary meningitis. J. Hosp. Infect. 40, 27–33 (1998)
S.C. Chang, W.C. Hsieh, C. Liu, High prevalence of antibiotic resistance of common pathogenic bacteria in Taiwan. The Antibiotics Resistance Study Group of the Infectious Disease Society of the Republic of China. Diagn. Microbiol. Infect. Dis. 36, 107–112 (2000)
M. Ho, L.C. McDonald, T.L. Lauderdale, L.L. Yeh, P.C. Chen, Y.R. Shiau, Surveillance of antibiotic resistance in Taiwan, 1998. J. Microbiol. Immunol. Infect. 32, 239–249 (1999)
J. Carlet, P. Collignon, D. Goldmann, H. Goossens, I.C. Gyssens, S. Harbarth et al., Society’s failure to protect a precious resource: antibiotics. Lancet 378, 369–371 (2011)
J. Carlet, J.L. Mainardi, Antibacterial agents: back to the future? Can we live with only colistine, cotrimoxazole, and fosfomycin? Clin. Microbiol. Infect. 18, 1–3 (2012)
A. De Leonardis, A. Aretini, G. Alfano, V. Macciola, G. Ranalli, Isolation of a hydroxytyrosol-rich extract from olive leaves (Olea europaea L.) and evaluation of its antioxidant properties and bioactivity. Eur. Food Res. Technol. 226, 653–659 (2008)
I.S. Arvanitoyannis, A. Kassaveti, Fish industry waste: treatments, environmental impacts, current and potential use. Int. J. Food Sci. Technol. 43, 726–745 (2008)
A. El-Abbassi, A. Hafidi, M.C. Garcia-Payo, M. Khayet, Concentration of olive mill wastewater by membrane distillation for polyphenols recovery. Desalination 245, 670–674 (2009)
E. Garcia-Castello, A. Cassano, A. Criscuoli, C. Conidi, E. Drioli, Recovery and concentration of polyphenols from olive mill wastewaters by integrated membrane system. Water Res. 44, 3883–3892 (2010)
O.A. Mudimu, M. Peters, F. Brauner, G. Braun, Overview of membrane processes for the recovery of polyphenols from olive mill wastewater. Am. J. Environ. Sci. 8(3), 195–201 (2012)
M. Larif, A. Zarrouk, A. Soulaymani, A. Elmidaoui, New innovation in order to recover the polyphenols of olive mill wastewater extracts for use as a biopesticide against the Euphyllura olivina and Aphis citricola. Res. Chem. Intermed. (2012). doi:10.1007/s11164-012-0947-5
C.M. Galanakis, E. Tornberg, V. Gekas, Clarificationofhigh-added value products from olive mill wastewater. J. Food Eng. 99, 190–197 (2010)
M.L. Cayuela, P.D. Millner, S.L.F. Meyer, A. Roig, Potential of olive mill waste and compost as biobased pesticides against weeds, fungi, and nematodes. Sci. Total Environ. 399, 11–18 (2008)
M. Larif, A. Soulaymani, M. Hnach, A. Elmidaoui, Olive wastewater’s impact on Oued Boufekrane in Meknes-Tafilalet. Am. J. Environ. Sci. 8, 236 (2012)
M. Larif, A. Elmidaoui, A. Zarrouk, H. Zarrok, R. Salghi, B. Hammouti, H. Oudda, F. Bentiss, An investigation of carbon steel corrosion inhibition in hydrochloric acid medium by an environmentally friendly green inhibitor. Res. Chem. Intermed. (2012). doi:10.1007/s11164-012-0788-2
A. Debo, T. Yangui, A. Dhouib, M. Ksantini, S. Sayadi, Efficacy of a hydroxytyrosol-rich preparation from olive mill wastewater for control of olive psyllid, Euphyllura olivina, infestations. Crop Prot. 30, 1529–1534 (2011)
V.L. Singleton, J.A. Rosi, Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid reagents. Am. J. Oenol. Vitic. 16, 144–158 (1965)
T. Hanato, H. Kagawa, T. Yasuhara, T. Okuda, Two new flavonoids and other constituents in licorice root: their relative relative astringency and radical scavenging effects. Chem. Pharm. Bull. 36, 2090–2097 (1988)
P.D. Duh, G.C. Yen, Antioxidant activity of water extract of Harng Jyur (Chrysanthemum morifolium Ramat.) varieties in soybean oil emulsion. Food Chem. 66, 471–476 (1999)
H. Falleh, R. Ksouri, K. Chaieb, N. Karray-Bouraoui, N. Trabelsi, M. Boulaaba, C. Abdelly, Phenolic composition of Cynara cardunculus L. organs, and their biological activities. C. R. Biol. 331, 372–379 (2008)
M. Larif, A. Adad, R. Hmammouchi, A. Idrissi Taghki, A. Soulaymani, A. Elmidaoui, M. Bouachrine, T. Lakhlifi, Biological activities of triazine derivatives. Combining DFT and QSAR results. Arab. J. Chem. (2013). doi:10.1016/j.arabjc.2012.12.033
Agence Française de Normalisation (Afnor), Recueil de normes Françaises: Eau, méthodes d’essai, 2nd edn. (Paris, France, 1983), p. 621
J.J. Macheix, A. Fleuriet, J.A. Billot, Fruit phenolics (CRC Press Inc., Boca Raton, 1990)
H.B. Li, K.W. Cheng, C.C. Wong, K.W. Fan, F. Chen, Y. Tian, Evaluation of antioxidant capacity and total phenolic content of different fraction of selected microalgae. Food Chem. 102, 771–776 (2007)
T. Bahorun, B. Grinier, F. Trotin, G. Brunet, T. Pin, M. Luncky, J. Vasseur, M. Cazin, C. Cazin, M. Pinkas, Oxygen species scavenging activity of phenolic extracts from hawthorn fresh plant organs and pharmaceutical preparations. Arzneimittelforschung 46(11), 1086–1089 (1996)
J. Rodier, L. Bernard, M. Nicole, coll. L’analyse de l’eau, eaux naturelles, eaux résiduaires, eaux de mer, 9ème édn. (Dunod, Paris, 2009). ISBN 987-2-10-054179-9
L. Gachkar, D. Yadegari, M.B. Rezaei, M. Taghizadeh, S.A. Astaneh, I. Rasooli, Chemical and biological characteristics of Cuminum cyminum and Rosmarinus officinalis essential oils. Food Chem. 102, 898–904 (2007)
G. Leyral, J.N. Joffin, Microbiologie technique: 2ème Édition., Collection Biologie technique. Pédagogique d’Aquitaine 2, 290 (2001)
C. Bekhech, F. Atik-Bekkara, E. Abdelouahid, F. Tomi, J. Casanova, Composition and antibacterial activity of the essential oil of Thymus fontanesii Boiss. Et Rent. from Algeria. J. Essent. Oil Res. 19, 594–596 (2007)
M. Bourkhiss, M. Hnach, B. Bourkhiss, M. Ouhssine, A. Chaouch, Composition chimique et propriétés antimicrobiennes de l’huile essentielle extraite des feuilles de Tetraclinis articulata (Vahl) du Maroc. Afrique Science 03(2), 232–242 (2007)
A. Remmal et al., Improved method of determination of antimicrobial activity of essential oils in agar medium. J. Essent. Oil Res. 5(2), 179–184 (1993)
B. Satrani et al., Composition chimique et activité antimicrobienne des huiles essentielles de Satureja calamintha et Satureja alpina du Maroc. Ann. Falsif. Expert. Chim. Toxicol. 94(956), 241–250 (2001)
C. Amaral, M.S. Lucas, J. Coutinho, L.A. Crespi, M.D.R. Anjos, C. Pais, Microbiological and physiochemical characterization of olive mill in Northeastern Portugal. Bioresour. Technol. 99, 7215–7223 (2008)
K. Loziene, P.R. Venskutonis, Influence of environmental and genetic factors on the stability of essential oil composition of Thymus pulegioides. Biochem. Syst. Ecol. 33, 517–525 (2006)
M.A. Curado et al., Environmental factors influence on chemical polymorphism of the essential oils of Lychnophora ericoides. Phytotherapy 67, 2363–2369 (2006)
A. Nefzaoui, Contribution à la rentabilité de l’oléiculture par une valorisation optimale des sous produits. Option méditerranéennes 16, 101–108 (1991)
A. Jail, E. Boukhoubza, A. Nejmeddine, S. Sayadi, L. Hassani, Co-treatment of olive-mill and urban wastewaters by experimental stabilization ponds. J. Hazard. Mater. 176, 893–900 (2010)
N. Leulmi, Etude de la valorisation nutritionnelle des margines et de leur impact sur la réduction de la méthanogènése ruminale chez l’ovin (Magister en Microbiologie appliquée, Nature du diplôme, 2011)
A.A. Aganga, K.W. Mosase, Tannins content, nutritivantee value and dry matter digestibility of Lonchocarous capussa, Ziziphus mucropata, Sclerocarya birrea, Kirkia acuminata and Rhus lancea seeds. Animal Feed Sci. Technol. 91, 107–113 (2001)
K. Pedneault, S. Leonhart, L. Angenol, A. Gosselin, A. Ramputh et J. T. Arnason. Influence de la culture hydroponique de quelques plantes médicinales sur la croissance et la concentration en composes secondaires des organes végétaux. Texte de conférence, 5ème colloque sur les produits naturels d’origine végétale, Université Laval, Quebec City, 1–5 (2001)
A. Yaacoubi, A. Chahlaoui, M. El yachioui, A. Chaouch, Traitement des margines à pH neutre et en conditions d’aérobie par la microflore du sol avant épandage. Bull. Soc. Pharm. Bordeaux. 149, 43–56 (2010)
A. Jouraiphy, S.M. El Gharous, J.C. Revel, M. Hafidi, Chemical and spectroscopic analysis of organic matter transformation during composting of sewage sludge and green plant waste. Int. Biodeterior. Biodegrad. 56, 101–108 (2005)
G. Leray, E. Vierling, Microbiologie et toxicologie des aliments: Hygiène et sécurité alimentaire, 4th edn. (CRDI d’Aquitaine, Malmaison Doin Bordeaux, 2007), p. 290
M. Hamdi, R. Ellouz, Treatment of detoxified olive mill wastewater by anaerobic filter and aerobic fluidized bed process. Environ. Technol. 19, 183–188 (1993)
A. Ranalli. L’effluent des huileries d’olive proposition en vue de son épuration, Références aux normes italiennes en la matière, première partie Olivia 37, 30–39 (1991)
A. Tantaoui-Elaraki, N. Lattaoui, A. Errifi, Composition and antimicrobial activity of the essential oil of Thymus broussonetii, T. Zygis and T. Satureoïde. J. Essent. Oil Res. 5, 45–53 (1993)
S. Takaç, A. Karakaya, Recovery of phenolic antioxidants from olive mill wastewater. Recent Pat. Chem. Eng. 2, 230–237 (2009)
A.P.A.D. Gurgel et al., Antibacterial effects of P. amboinicus (Lour.) Spreng (Lamiaceae) in methicillin resistant S. aureus (MRSA). Biol. Res. 3(3–4), 117–122 (2009)
G. Ciafardini, B.A. Zullo, Antibacterial activity of oil mill water polyphenols on the phytopathogen Xanthomonas campestris spp. Ann. Microbiol. 53, 283–290 (2003)
A. Sousa, I.C.F.R. Ferriara, R. Calhella, P.B. Andrade, P. Valentao, R. Seabra et al., Phenolics and antimicrobial activity of traditional stoned table olives alcaparra. Bioorg. Med. Chem. 14, 8533–8538 (2006)
D. Karou, M. H. Dicko, J. Simporé, S. Yameogo, S. Sanon et A. S. Traoré. Activités antioxydantes et antibactériennes des polyphénols extraits de plantes medicinales de la pharmacopée traditionnelle du Burkina Faso. Maitrise des procédés en vue d’améliorer la qualité des aliments, utilisation des OGM, analyse des risques en agroalimentaire. 8–11 novembre. Ouagadougou (2005)
A. Firas, F. Hassan, Antibacterial and antifungal activities of different parts of Tribulus terrestris L. growing in Iraq. J. Zhejiang Univ. Sci. 9(2), 154–159 (2008)
A. Dhiman, A. Nanda, A. Sayeed, A quest for staunch effects of flavonoids: Utopian protection against hepatic ailments. Arab. J. Chem. (2012). doi:10.1016/j.arabjc.2012.05.001
M.M. Cowan, Plant products as antimicrobial agents. Clin. Microbiol. Rev. 12, 564–582 (1999)
A. Zhiri, D. Baudoux, Huiles essentielles chémotypées et leurs synergies: aromathérapie scientifique (Édition Inspir Development, Luxembourg, 2005)
M. Viuda-Martos, Y. Ruiz-Navajas, J. Fernández-López, J. Perez-Álvarez, Antibacterial activity of lemon (Citrus lemon), mandarin (Citrus reticulata), grapefruit (Citrus paradisi) and orange (Citrus sinensis) essential oils. J. Food Saf. 28(4), 567–576 (2008)
T. Beta, S. Nam, J.E. Dexter, H.D. Sapirstein, Phenolic content and antioxidant activity of pearled wheat and Roller-Milled fractions. Cereal Chem. 82, 390–393 (2005)
S. Omar Said Hassane, B. Satrani, M. Ghanmi, N. Mansouri, H. Mohamed, A. Chaouch, Activité antimicrobienne et composition chimique de l’huile essentielle de Plectranthus aromaticus Roxb. de l’Ile de la Grande Comore. Biotechnol. Agron. Soc. Environ. 15(2), 251–258 (2011)
C.Y. Gaudreau, H. Jilbert, I. Josée Gagnon, S. Bekal, Comparison of disk diffusion and agar dilution methods for erythromycin, ciprofloxacin, and tetracycline susceptibility testing of Campylobacter coli and for tetracycline susceptibility testing of Campylobacter jejuni subsp. Jejuni Antimicrob Agents Chemother 52(12), 4475–4477 (2008)
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Larif, M., Ouhssine, M., Soulaymani, A. et al. Potential effluent oil mills and antibacterial activity polyphenols against some pathogenic strains. Res Chem Intermed 41, 1213–1225 (2015). https://doi.org/10.1007/s11164-013-1267-0
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DOI: https://doi.org/10.1007/s11164-013-1267-0