Abstract
Plants growing under an increased radiation background may be exposed to additional stressors. Plant acclimatization is formed with the participation of stress signals that cause systemic responses—a change in the activity of physiological processes. In this work, we studied the mechanisms of the effect of ionizing radiation (IR) on the systemic functional responses induced by electrical signals. Chronic β-irradiation (31.3 μGy/h) have a positive effect on the morphometric parameters and photosynthetic activity of tobacco plants (Nicotiana tabacum L.) at rest. An additional stressor causes an electrical signal, which, when propagated, causes a temporary change in chlorophyll fluorescence parameters, reflecting a decrease in photosynthesis activity. Irradiation did not significantly affect the electrical signals. At the same time, more pronounced photosynthesis responses are observed in irradiated plants: both the amplitude and the leaf area covered by the reaction increase. The formation of such responses is associated with changes in pH and stomatal conductance, the role of which was analyzed under IR. Using tobacco plants expressing the fluorescent pH-sensitive protein Pt-GFP, it was shown that IR enhances signal-induced cytoplasmic acidification. It was noted that irradiation also disrupts the correlation between the amplitudes of the electrical signal, pH shifts, changes in chlorophyll fluorescence parameters. Also stronger inhibition of stomatal conductance by the signal was shown in irradiated plants. It was concluded that the effect of IR on the systemic response induced by the electrical signal is mainly due to its effect on the stage of signal transformation into the response.
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This work was supported by Russian Science Foundation (Grant No. 23-24-00340).
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MG and VV: conceptualization; MG, AB and VV: methodology; MG, YN and VV: formal analysis; MG, YN and MA: investigation; AB and VV: resources; AB and VV: data curation; MG: writing - original draft; VV: writing—review & editing; MG, MA and VV: visualization; VV: supervision; VV: funding acquisition. All authors read and approved the final manuscript.
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Grinberg, M., Nemtsova, Y., Ageyeva, M. et al. Effect of low-dose ionizing radiation on spatiotemporal parameters of functional responses induced by electrical signals in tobacco plants. Photosynth Res 157, 119–132 (2023). https://doi.org/10.1007/s11120-023-01027-9
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DOI: https://doi.org/10.1007/s11120-023-01027-9