Abstract
The emission spectra of CP47-RC and core complexes of Photosystem II (PS II) were measured at different temperatures and excitation wavelengths in order to establish the origin of the emission and the role of the core antenna in the energy transfer and charge separation processes in PS II. Both types of particles reveal strong dependences of spectral shape and yield on temperature. The results indicate that the well-known F-695 emission at 77 K arises from excitations that are trapped on a red-absorbing CP47 chlorophyll, whereas the F-685 nm emission at 77 K arises from excitations that are transferred slowly from 683 nm states in CP47 and CP43 to the RC, where they are trapped by charge separation. We conclude that F-695 at 77 K originates from the low-energy part of the inhomogeneous distribution of the 690 nm absorbing chlorophyll of CP47, while at 4 K the fluorescence originates from the complete distribution of the 690 nm chlorophyll of CP47 and from the low-energy part of the inhomogeneous distribution of one or more CP43 chlorophylls.
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EG Andrizhiyevskaya D Frolov R Grondelle Particlevan JP Dekker (2004) ArticleTitleOn the role of CP47 antenna in energy transfer and trapping in Photosystem II Phys Chem Chem Phys 6 4810–4819 Occurrence Handle10.1039/b411977k
JP Dekker EJ Boekema (2005) ArticleTitleSupramolecular organization of thylakoid membrane proteins in green plants Biochim Biophys Acta 1706 12–39 Occurrence Handle15620363
JP Dekker R Grondelle Particlevan (2000) ArticleTitlePrimary charge separation in Photosystem II Photosynth Res 63 195–208 Occurrence Handle10.1023/A:1006468024245
JP Dekker NR Bowlby CF Yocum (1989) ArticleTitleChlorophyll and cytochrome-b-559 content of the photochemical reaction center of Photosystem II FEBS Lett 254 150–154 Occurrence Handle10.1016/0014-5793(89)81028-2
KN Ferreira TM Iverson K Maghlaoui J Barber S Iwata (2004) ArticleTitleArchitecture of the photosynthetic oxygen-evolving center Science 303 1831–1838 Occurrence Handle14764885
M-L Groot EJG Peterman PJM Kan Particlevan IHM Stokkum Particlevan JP Dekker R Grondelle Particlevan (1994) ArticleTitleTemperature dependent triplet and fluorescence quantum yields of the photosystem II reaction center described in a thermodynamic model Biophys J 67 318–330 Occurrence Handle7919002
M-L Groot EJG Peterman IHM Stokkum Particlevan JP Dekker R Grondelle Particlevan (1995) ArticleTitleTriplet and fluorescing states of the CP47 antenna complex of photosystem II studied as a function of temperature Biophys J 68 281–290 Occurrence Handle7711252
M-L Groot RN Frese FL Weerd Particlede K Bromek Å Pettersson EJG Peterman IHM Stokkum R Grondelle JP Dekker (1999) ArticleTitleSpectroscopic properties of the CP43 core antenna protein of photosystem II Biophys J 77 3328–3340 Occurrence Handle10585955
B Hillmann K Brettel F Mieghem ParticleVan A Kamlowski AW Rutherford E Schlodder (1995) ArticleTitleCharge recombination reactions in Photosystem II. 2. Transient absorbance difference spectra and their temperature dependence Biochemistry 34 4814–4827 Occurrence Handle7718588
N Kamiya J-R Shen (2003) ArticleTitleCrystal structure of oxygen-evolving photosystem II from Thermosynechococcus vulcanus at 3.7 Å resolution Proc Natl Acad Sci USA 100 98– 103 Occurrence Handle12518057
Krausz E, Hughes JL, Smith PJ, Pace RJ and Peterson Årsköld S (2005) Assignment of the low-temperature fluorescence in oxygen-evolving photosystem II. Photosynth Res, in press
MA Palacios Weerd FL de Ihalainen JA R Grondelle Particlevan H Amerongen Particlevan (2002) ArticleTitleSuperradiance and exciton (de)localization in light-harvesting Complex II from green plants? J Phys Chem B 106 5782–5787
LO Pålsson C Flemming B Gobets R Grondelle Particlevan JP Dekker E Schlodder (1998) ArticleTitleEnergy transfer and charge separation in photosystem I: P700 oxidation upon selective excitation of the long-wavelength antenna chlorophylls of Synechococcus elongates Biophys J 74 2611–2622 Occurrence Handle9591685
EJG Peterman H Amerongen ParticleVan R Grondelle Particlevan JP Dekker (1998) ArticleTitleThe nature of the excited state of the reaction center of Photosystem II of green plants. A high-resolution fluorescence spectroscopy study Proc Natl Acad Sci USA 95 6128–6133 Occurrence Handle9600929
M Rögner PJ Nixon BA Diner (1990) ArticleTitlePurification and characterization of photosystem I and photosystem II core complexes from wild-type and phycocyanin-deficient strains of the cyanobacterium Synechocystis PCC 6803 J Biol Chem 265 6189–96 Occurrence Handle2108153
GH Schatz H Brock AR Holzwarth (1988) ArticleTitleKinetic and energetic model for the primary processes in photosystem II Biophys J 54 397–405
X-S Tang BA Diner (1994) ArticleTitleBiochemical and spectroscopic characterization of a new oxygen-evolving Photosystem II core complex from the cyanobacterium synechocystis PCC 6803 Biochemistry 33 4594–603 Occurrence Handle8161515
RJ Dorssen ParticleVan JJ Plijter JP Dekker A den Ouden J Amesz HJ Gorkom (1987a) ArticleTitleSpectroscopic properties of chloroplast grana membranes and of the core of Photosystem II Biochim Biophys Acta 890 134–143
RJ Dorssen ParticleVan J Breton JJ Plijter K Satoh HJ Gorkom Particlevan J Amesz (1987b) ArticleTitleSpectroscopic properties of the reaction center and of the 47 kDa chlorophyll protein of Photosystem II Biochim Biophys Acta 893 267–274
Grondelle Van (1985) ArticleTitleExcitation energy transfer, trapping and annihilation in photosynthetic systems Biochim Biophys Acta 811 147–195
PJ Van Leeuwen MC Nieveen EJ Meent Particlevan de JP Dekker HJ Gorkom (1991) ArticleTitleRapid and simple isolation of pure photosystem II core and reaction center particles from spinach Photosynth Res 28 149–153 Occurrence Handle10.1007/BF00054128
F Van Mourik M-L Groot R Grondelle JP Dekker IHM Stokkum Particlevan (2004) ArticleTitleGlobal and target analysis of fluorescence measurements on Photosystem 2 reaction centers upon red excitation Phys Chem Chem Phys 6 4820–4824 Occurrence Handle10.1039/b407633h
A Zouni HT Witt J Kern P Fromme N Krauß W Saenger P Orth (2001) ArticleTitleCrystal structure of photosystem II from Synechococcus elongatus at 3.8 Å resolution Nature 409 739–743 Occurrence Handle11217865
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Andrizhiyevskaya, E.G., Chojnicka, A., Bautista, J.A. et al. Origin of the F685 and F695 fluorescence in Photosystem II. Photosynth Res 84, 173–180 (2005). https://doi.org/10.1007/s11120-005-0478-7
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DOI: https://doi.org/10.1007/s11120-005-0478-7