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Rapid and simple isolation of pure photosystem II core and reaction center particles from spinach

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Abstract

Pure and active oxygen-evolving PS II core particles containing 35 Chl per reaction center were isolated with 75% yield from spinach PS II membrane fragments by incubation with n-dodecyl-β-D-maltoside and a rapid one step anion-exchange separation. By Triton X-100 treatment on the column these particles could be converted with 55% yield to pure and active PS II reaction center particles, which contained 6 Chl per reaction center.

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Abbreviations

Bis-Tris:

bis[2-hydroxyethyl]imino-tris[hydroxymethyl]methane

Chl:

chlorophyll

CP29:

Chl a/b protein of 29 kDa

Cyt b 559 :

cytochrome b 559

DCBQ:

2,5-dichloro-p-benzo-quinone

LHC II:

light-harvesting complex II, predominant Chl a/b protein

MES:

2-[N-Morpholino]ethanesulfonic acid

Pheo:

pheophytin

PS H:

photosystem II

QA :

bound plastoquinone, serving as the secondary electron acceptor in PS II (after Pheo)

SDS:

sodiumdodecylsulfate

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Authors and Affiliations

  1. Department of Biophysics, Huygens laboratory of the State University, P.O. Box 9504, 2300 RA, Leiden

    Peter J. van Leeuwen, Maaike C. Nieveen, Erik Jan van de Meent & Hans J. van Gorkom

  2. Department of Physics and Astronomy, Free University, De Boelelaan 1081, 1081 HV, Amsterdam, the Netherlands

    Jan P. Dekker

Authors
  1. Peter J. van Leeuwen
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  2. Maaike C. Nieveen
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  3. Erik Jan van de Meent
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  4. Jan P. Dekker
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  5. Hans J. van Gorkom
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van Leeuwen, P.J., Nieveen, M.C., van de Meent, E.J. et al. Rapid and simple isolation of pure photosystem II core and reaction center particles from spinach. Photosynth Res 28, 149–153 (1991). https://doi.org/10.1007/BF00054128

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  • DOI: https://doi.org/10.1007/BF00054128

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