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Phylogenetic and Expression Analysis of Pear Yellow Stripe-Like Transporters and Functional Verification of PbrYSL4 in Pear Pollen

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Abstract

Yellow stripe-like (YSL) family transporters, belonging to the oligopeptide transporter family, are significant iron transport proteins. In this study, we provided a genome-wide identification and analysis of the YSL gene family in Pyrus bretschneideri. We found eight YSL gene members in pear, clustered into four main groups in the phylogenetic tree. Segmental duplication has played a key role in the expansion of the pear YSL family. The pollen activity analysis indicated that the low concentration of iron ion was beneficial to both pear pollen germination and pollen tube growth. Among the eight YSL genes, PbrYSL4 had particularly high expression in all pear tissues; it was significantly responsive to change in the external iron ion supply in the pollen cultivation in vitro. Moreover, expression of PbrYSL4 in yeast mutant Δccc1 (Ca 2+ -sensitive cross-complementer 1 mutant) made Δccc1 restore growth in high iron medium. These data together suggest that PbrYSL4 was involved in the movement of iron in the pear pollen tube growth.

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Abbreviations

BLAST:

Base local alignment search tool

CDS:

Coding sequence

DMA:

Deoxymugineic acid

MEME:

Multiple EM for motif elicitation

MES:

2-(N-morpholino) ethanesulfonic acid hydrate

ML:

Maximum likelihood

NA:

Nicotianamine

RAP:

Rice genome annotation project

YSL:

Yellow stripe-like

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Acknowledgments

This work was supported by the National Key Technology R&D Program of the Ministry of Science and Technology of China (2014BAD16B03-4), the Independent Innovation of Agricultural Sciences in Jiangsu Province [CX(13)3010, CX(12)5079 and CX(14)2020], the National Natural Science Foundation of China (31522048, 31272119, 31171936), Jiangsu Province Science and Technology Support Program (BE2014400 and BE2014334) and the Doctoral Fund of Ministry of Education of China (20110097110029, 20120097120046, 20120097110041 and 20130097130004). We thank International Science Editing for helping us to improve the article.

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    Authors and Affiliations

    1. Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, 210095, China

      Jie Yang, Jianqing Chen, Peng Wang, Musana R. Fabrice, Shaoling Zhang & Juyou Wu

    2. Department of Biochemistry and Molecular Biology, College of Life Science, Nanjing Agricultural University, Nanjing, 210095, China

      Xi Chen & Gang Ma

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    1. Jie Yang
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    Correspondence to Juyou Wu.

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    Jie Yang and Jianqing Chen contributed equally to this work.

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    Yang, J., Chen, J., Chen, X. et al. Phylogenetic and Expression Analysis of Pear Yellow Stripe-Like Transporters and Functional Verification of PbrYSL4 in Pear Pollen. Plant Mol Biol Rep 34, 737–747 (2016). https://doi.org/10.1007/s11105-015-0959-6

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