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Identification and Expression Pattern of a ZPR1 Gene in Wild Tomato (Solanum Pennellii)

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Abstract

The mammalian zinc finger protein ZPR1 is known to be essential in cell cycle progression, but its homolog in plants remains to be identified. Here, we isolated SpZPR1 from the wild tomato species Solanum pennellii LA0716 and found that its protein sequence shares homology with ZPR1 genes from other organisms. SpZPR1 mRNA was found to accumulate in tomato apical meristem in response to ABA (abscisic acid) treatment and heat stress. A transactivation activity assay in yeast revealed that SpZPR1 functions as a transcriptional activator, while the results of our gel electrophoresis mobility shift assay showed that SpZPR1 was able to bind the ABRE (ABA-responsive element) sequence in vitro. Our study is the first to provide evidence that SpZPR1 is involved in the ABA signaling network and plays a potential role in plant cell development and abiotic stress response.

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Acknowledgments

We thank Dr. Khurram Ziaf for critical reviews of this manuscript. This work was supported by grants from Project 973 (NO. 2009CB119002), the National High Technology R&D Program of China (2012AA100104) and the National Natural Science Foundation of China (30871712).

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Authors and Affiliations

  1. Key Laboratory of Horticultural Plant Biology, MOE, Huazhong Agricultural University, Wuhan, 430070, China

    Jinhua Li, Wei Sima, Bo Ouyang, Zhidan Luo, Changxian Yang, Zhibiao Ye & Hanxia Li

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  1. Jinhua Li
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  2. Wei Sima
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  3. Bo Ouyang
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  4. Zhidan Luo
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  5. Changxian Yang
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  7. Hanxia Li
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Correspondence to Hanxia Li.

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Supplementary Fig. S1

Full-length cDNA sequence of the SpZPR1 gene and the deduced amino acid sequence. (DOC 26 kb)

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Li, J., Sima, W., Ouyang, B. et al. Identification and Expression Pattern of a ZPR1 Gene in Wild Tomato (Solanum Pennellii). Plant Mol Biol Rep 31, 409–417 (2013). https://doi.org/10.1007/s11105-012-0509-4

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