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Overexpression of a Transcription Factor OsMADS15 Modifies Plant Architecture and Flowering Time in Rice (Oryza sativa L.)

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Abstract

The MADS-box gene family encodes conserved transcription factors and functions not only in reproductive development, but also in vegetative growth. In this study, a rice MADS-box gene OsMADS15, an ortholog of Arabidopsis AP1 gene, has been functionally characterized. Rice seedlings overexpressing OsMADS15 showed precocious phenotypes of early internode elongation, shoot-borne crown root development, reduced plant height and early flowering. The axillary buds developments in OsMADS15 overexpressors were accelerated, and the buds frequently grew into effective tillers. The panicles of OsMADS15 transgenic rice plants were largely compromised for growth and branching in comparison with wild type. In the tillering stage, the OsMADS15 overexpression rice plants tillered later and less than the wild type, and in the maturity stage, the culms of the overexpression lines bore more stem nodes. Quantitative polymerase chain reaction (PCR) results showed that expression levels of WUSCHEL-related Homeobox (WOX) gene, WOX11, and some flowering regulators were promoted in the OsMADS15 overexpression transgenic plants, indicating that OsMADS15 had a wide range of regulations. These results clearly indicate that OsMADS15 plays important roles not only in transition to reproductive development, but also in crown root development.

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Acknowledgements

We are very grateful to Prof. Jian-Xiang Liu in Fudan University for his help in manuscript preparation. This work was supported by the “863” Research Program of China (2006AA10Z159).

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  1. Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Yuhangtang Road 866, Hangzhou, 310058, China

    Sun-Jie Lu, He Wei, Ya Wang, Hui-Mei Wang, Rui-Fang Yang, Xiao-Bo Zhang & Ju-Min Tu

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  1. Sun-Jie Lu
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  2. He Wei
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  3. Ya Wang
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Correspondence to Ju-Min Tu.

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S.-J. Lu, H. Wei and Y. Wang contributed equally to this work.

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Lu, SJ., Wei, H., Wang, Y. et al. Overexpression of a Transcription Factor OsMADS15 Modifies Plant Architecture and Flowering Time in Rice (Oryza sativa L.). Plant Mol Biol Rep 30, 1461–1469 (2012). https://doi.org/10.1007/s11105-012-0468-9

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