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Characterization of the LhcSR Gene Under Light and Temperature Stress in the Green Alga Ulva linza

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Abstract

As a green-tide-forming macroalga, Ulva linza is distributed worldwide and therefore subject to various environmental stresses. The LHCSR (also known as LI818 in green alga and LHCX in diatoms) protein is a stress-related member of the LHC family that plays an important role in photo-protective mechanism, which has been only found in algae. In this study, we cloned full-length cDNA sequence encoding the LhcSR gene from U. linza and analyzed its expression in response to different temperature and illumination gradients. The results showed that high light (HL) could enhance expression of LhcSR and that the expression level peaked at 3 h under HL. Similarly, the expression of LhcSR could also be induced by low temperature (LT). However, the expression patterns of LhcSR were quite different in response to LT and HL treatment. Specifically, the maximum gene expression under LT was much higher (11.8-fold) than under HL (5.4-fold) when compared to the expression under normal conditions. The upregulated expression of LhcSR lasted for 12 h under LT, but 3 h under HL. These data suggest that the LhcSR gene is involved in photoprotection in U. linza, and the results suggest a stronger link to LT. In addition, the discrepancy in expression under HL and LT was consistent with the ecological features of this alga, which only thrives during the cold season (featured as LT and low light).

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Acknowledgments

This work was supported by National special fund for transgenic project (2009ZX08009-019B), the Hi-Tech Research and Development Program (863) of China (2009AA10Z106), Natural Science Foundation of Shandong Province (2009ZRA02075), Qingdao Municipal Science and Technology plan project (09-2-5-8-hy, 10-4-1-13-hy) and the National Science & Technology Pillar Program (2008BAD95B11).

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Authors and Affiliations

  1. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China

    Meitao Dong, Xiaowen Zhang, Zhimeng Zhuang, Jian Zou, Naihao Ye & Dong Xu

  2. Qingdao Agricultural University, Qingdao, 266109, China

    Meitao Dong & Wenqi Wang

  3. Key Laboratory of Marine Bioactive substance, The First Institute of Oceanography, State Oceanic Administration (SOA), Qingdao, 266061, China

    Shanli Mou

  4. Qingdao University of Science & Technology, Qingdao, 266042, China

    Chengwei Liang

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  1. Meitao Dong
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Correspondence to Naihao Ye or Wenqi Wang.

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Dong, M., Zhang, X., Zhuang, Z. et al. Characterization of the LhcSR Gene Under Light and Temperature Stress in the Green Alga Ulva linza . Plant Mol Biol Rep 30, 10–16 (2012). https://doi.org/10.1007/s11105-011-0311-8

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