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Cloning and Characterization of a Stearoyl–Acyl Carrier Protein Desaturase Gene from Cinnamomum longepaniculatum

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Abstract

The stearoyl–acyl carrier protein desaturase (SAD) is a key enzyme that determines the ratio of saturated to unsaturated fatty acids in higher plants. Using reverse transcriptase polymerase chain reaction and rapid amplification of cDNA ends, a full-length cDNA of SAD was obtained from developing leaves of Cinnamomum longepaniculatum. Sequence analysis showed that the deduced amino acid sequence had high similarity to other reported SADs. The CSAD gene was functionally expressed in Escherichia coli, and the desaturating activity of the recombinant protein was markedly detected when assayed in vitro by adding spinach ferredoxin. Southern blot analysis indicated that the gene was a member of a small gene family. Northern blotting revealed that the CSAD gene was highly expressed in developing leaves of C. longepaniculatum. These results would provide opportunities for modifying fatty acid composition in C. longepaniculatum.

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Abbreviations

CSAD:

stearoyl–acyl carrier protein (ACP) desaturase from Cinnamomum longepaniculatum

pQESAD:

vector pQE30 with CSAD

SAD:

stearoyl–acyl carrier protein (ACP) desaturase

References

  • Davydov R, Behrouzian B, Smoukov S, Stubbe J, Hoffman BM, Shanklin J. Effect of substrate on the diiron (III) site in stearoyl acyl carrier protein delta 9-desaturase as disclosed by cryoreduction electron paramagnetic resonance/electron nuclear double resonance spectroscopy. Biochemistry 2005;44:1309–15.

    Article  PubMed  CAS  Google Scholar 

  • Fox BG, Shanklin J, Ai J, Loehr TM, Sanders-Loehr J. Resonance Raman evidence for a Fe-O-Fe center in stearoyl-ACP desaturase primary sequence identity with other diiron-oxo proteins. Biochemistry 1994;33:12776–86.

    Article  PubMed  CAS  Google Scholar 

  • Knutzon DS, Scherer DE, Schreckengost WE. Nucleotide sequence of a complementary DNA clone encoding stearoyl-acyl carrier protein desaturase from castor bean, Ricinus communi. Plant Physiol 1991;96:344–5.

    Article  PubMed  CAS  Google Scholar 

  • Knutzon DS, Thompson GA, Radke SE, Johnson WB, Knauf VC, Kridl JC. Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene. Proc Natl Acad Sci U S A 1992;89:2624–8.

    Article  PubMed  CAS  Google Scholar 

  • Kodama H, Horiguchi G, Nishiuchi T, Nishmura M, Iba K. Fatty acid desaturation during chilling acclimation is one of the factors involved in conferring low-temperature tolerance to young tobacco leaves. Plant Physiol 1995;109:1177–85.

    Google Scholar 

  • Lindqvist Y, Huang W, Schneider G, Shanklin J. Crystal structure of Δ9 stearoyl-acyl carrier protein desaturase from castor seed and its relationship to other di-iron proteins. EMBO J 1996;15:4081–92.

    PubMed  CAS  Google Scholar 

  • Los DA, Murata N. Structure and expression of fatty acid desaturase. Biochim Biophys Acta 1998;1394:3–15.

    PubMed  CAS  Google Scholar 

  • Luo ZJ, Li WY, Wei Q, Luo T. The status quo and future of Cinnamomum longepaniculatum in Yibin. J Sichuan Norm Uni (Nat Sci) 2001;24:317–9.

    CAS  Google Scholar 

  • Madi L, Wang X, Kobiler I, Lichter A, Prusky D. Stress on avocado fruits regulates Δ9- stearoyl ACP desaturase expression, fatty acid composition, antifungal diene level and resistance to Colletotrichum gloeosporioides attack. Physiol Mol Plant Pathol 2003;62:277–83.

    Article  CAS  Google Scholar 

  • Sambrook J, Fritsch EF, Miniatis T. Molecular cloning: a laboratory manual. 2nd ed. New York: Cold Spring Harbor; 1989.

    Google Scholar 

  • Shah FH, Rashid O, San CT. Temporal regulation of two isoforms of cDNA clones encoding delta 9-stearoyl-ACP desaturase from oil palm (Elaies guineensis). Plant Sci 2000;152:27–33.

    Article  CAS  Google Scholar 

  • Thompson GA, Scherer DE, Foxall-Van Aken S, Kenny JW, Young HL, Shintani DK, et al. Primary structures of the precursor and mature forms of stearoyl-acyl carrier protein desaturase from safflower embryos and requirement of ferredoxin for enzyme activity. Proc Natl Acad Sci U S A 1991;88:2578–82.

    Article  PubMed  CAS  Google Scholar 

  • Zaborowska Z, Starzycki M, Femiak I, Swiderski M, Legocki AB. Yellow lupine gene encoding stearoyl-ACP desaturase-organization, expression and potential application. Acta Biochim Pol 2002;49:29–42.

    PubMed  CAS  Google Scholar 

  • Zhang NH, Wei ZQ, He JX, He JX, Du LF, Liang HG, et al. An efficient and economic method for preparation of high quality plant RNA. Prog Biochem Biophys 2004;31:947–50.

    CAS  Google Scholar 

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Acknowledgments

This work was supported by Key Science and Technology Program of Yibin City (200503016), Key Program of Education Department of Sichuan Province (07ZA146), and Doctor Startup Program of Yibin University (2006B02).

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Authors and Affiliations

  1. Department of Life Sciences and Food Engineering, Yibin University, 8th Jiusheng Road, Wuliangye Av., Yibin City, Sichuan Province, 644007, People’s Republic of China

    Tong Luo, Wu Yuan Deng & Jin Zeng

  2. College of Life Sciences, Sichuan University, Chengdu, 610064, People’s Republic of China

    Fu Li Zhang

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  1. Tong Luo
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  2. Wu Yuan Deng
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  3. Jin Zeng
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  4. Fu Li Zhang
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Correspondence to Tong Luo.

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Luo, T., Deng, W.Y., Zeng, J. et al. Cloning and Characterization of a Stearoyl–Acyl Carrier Protein Desaturase Gene from Cinnamomum longepaniculatum . Plant Mol Biol Rep 27, 13–19 (2009). https://doi.org/10.1007/s11105-008-0046-3

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  • DOI: https://doi.org/10.1007/s11105-008-0046-3

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