Abstract
Cucumber mosaic virus Ca-P1 strain, a P0 resistance-breaking virus, was isolated from the leaves of virus-infected Capsicum annuum ‘Manidda’ (a P0 resistant cultivar) in South Korea. Since CMV-Ca-P1 was first reported in 2006, this virus causing damage on pepper production has been constantly detected in South Korea. We constructed three CMV RNAi vectors based on the post-transcriptional gene silencing of defense system against virus infection. These independent vectors (hpCMV1, hpCMV2, and hpCMV2 + 1 RNAi vectors) were designed to produce dsRNAs containing each hpCMV1 (1270–1629 nt), hpCMV2 (1–300 nt), and hpCMV2 + 1 (fused hpCMV2 and hpCMV1) fragments, in RNA-1 (replicase gene) of CMV-Ca-P1, which were then confirmed by Agrobacterium-mediated transformation transient assay. Among these, dsRNAs expressed from the hpCMV2 + 1 vector showed resistance to both CMV-Ca-P1 and CMV-Fny (ordinary strain). To obtain high level of resistance to both CMV-Ca-P1 and CMV-Fny, transgenic Nicotiana benthamiana plants containing hpCMV2 + 1 vector were developed and conferred resistance to both CMV-Ca-P1 and CMV-Fny. This study contributes to the effective selection of target sequences that may inhibit CMV infection.
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Acknowledgements
We thank Dr. Mi Yeon Lee (University of California, Berkeley, California, USA) for helpful advice. This study was supported in part by Grants (2014M3A9B8022821, 2015R1D1A1A01060614) from the National Research Foundation in Korea.
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Song, E.G., Ryu, K.H. Engineering resistance to a resistance-breaking strain of Cucumber mosaic virus in plants utilizing viral dsRNA. Plant Biotechnol Rep 11, 429–438 (2017). https://doi.org/10.1007/s11816-017-0461-8
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DOI: https://doi.org/10.1007/s11816-017-0461-8