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Soilborne glyphosate residue thresholds for wheat seedling metabolite profiles and fungal root endophyte colonisation are lower than for biomass production in a sandy soil

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Abstract

Background and aims

Repeated applications of glyphosate in short timeframes can lead to periodic accumulation of soilborne glyphosate residues. This study aimed to determine whether typical glyphosate residues observed in fields affect growth, plant metabolite composition and fungal root endophyte colonisation of wheat (Triticum aestivum).

Methods

Glyphosate was applied to a sandy soil at 0, 0.33, 1, 3, 9 and 27 times a recommended label rate (LR) to establish soil glyphosate concentrations of 0, 0.1, 0.4, 1, 4.7 and 14.8 mg kg−1 in topsoil (0–100 mm) after a 28 d plant-free incubation. Wheat plants were then grown to establish thresholds of soilborne glyphosate on seedling emergence, major alterations to plant metabolic responses and fungal root endophyte colonisation.

Results

Seedling emergence was unaffected by soil residual glyphosate treatments. Shoot biomass was significantly higher at 1 mg kg−1 compared to the control treatments (P < 0.05) and root biomass followed a similar trend. Leaf metabolite profiles of plants growing in soil containing 4.7–14.8 mg kg−1 glyphosate could be discriminated from the 0–0.4 mg kg−1 treatments due to significant (P < 0.05) effects on the relative concentrations of metabolites. Fungal root endophyte colonisation was significantly reduced to 10% at 14.8 mg kg−1 (P < 0.05) whereas shoot and root biomass remained unaffected.

Conclusions

Our study highlights differences in glyphosate sensitivity thresholds between plants and fungal symbionts. Sub-lethal concentrations of residual soilborne glyphosate can alter wheat metabolism and impair fungal root endophyte colonisation. Effect thresholds for glyphosate may be lower for fungal symbionts than for host plants. However, the soil glyphosate residue levels required to reach such thresholds are high and would only be approached in commercial fields with a history of repeated glyphosate application.

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Authors and Affiliations

  1. Southern Cross Plant Science, Southern Cross University, Lismore, NSW, 2480, Australia

    Anders Claassens, Lukas Van Zwieten & Terry J. Rose

  2. NSW Department of Primary Industries, Wollongbar, NSW, 2477, Australia

    Anders Claassens, Michael T. Rose & Lukas Van Zwieten

  3. Centre for AgriBioscience, Latrobe University, Melbourne, Victoria, 3086, Australia

    Zhe (Han) Weng

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  1. Anders Claassens
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  2. Michael T. Rose
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  3. Lukas Van Zwieten
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  4. Zhe (Han) Weng
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Correspondence to Anders Claassens.

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Supplementary Table 1

Normalised peak areas of detected metabolites in wheat tissues under grown under different doses of soilborne glyphosate residues (XLSX 51 kb)

Supplementary Table 2

ANOVA results for influential metabolites contributing high loadings to PLS-DA components 1 and 2 (XLSX 13 kb)

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Claassens, A., Rose, M.T., Van Zwieten, L. et al. Soilborne glyphosate residue thresholds for wheat seedling metabolite profiles and fungal root endophyte colonisation are lower than for biomass production in a sandy soil. Plant Soil 438, 393–404 (2019). https://doi.org/10.1007/s11104-019-04023-y

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