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Stimulation of phosphorus uptake by ammonium nutrition involves plasma membrane H+ ATPase in rice roots

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Abstract

Aims

Nitrogen, especially NH +4 , can stimulate the uptake of phosphorus in plants, but the underlying mechanisms have not been clearly elucidated. Because phosphate is taken up via an anion/H+ co-transport process, we propose that the stimulated uptake of phosphorus by NH +4 versus NO -3 nutrition may be related to the activity of plasma membrane H+ ATPase. In the present study, we investigated the effect of NH +4 and NO -3 on phosphorus uptake and plasma membrane H+ ATPase activity in rice.

Methods

Rice plants were cultivated in a hydroponic solution with NH +4 or NO -3 . After 15 days of cultivation, phosphorus content was determined. Root plasma membrane was isolated using a two-phase partitioning system and hydrolytic H+-ATPase activity was determined by measuring the Pi concentration after a 30-min hydrolysis reaction. Relative expression of plasma membrane H+ ATPase genes was analyzed by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). H+ ATPase enzyme concentration in the plasma membrane was detected by western blot. For 33P uptake experiments, rice roots were incubated in the nutrient solution with addition of H 333 PO4.

Results

P content in both the roots and shoots of rice plants supplemented with NH +4 was significantly higher than P content in plants grown with NO -3 . Plasma membrane H+ ATPase activity in NH +4 -fed rice roots was significantly higher than that in NO -3 -fed rice roots. Real-time qRT-PCR and western blot results indicated that the higher activity of plasma membrane H+ ATPase in NH +4 -fed rice roots could be attributed to increased expression of the OSA1, OSA3, OSA7, OSA8 and OSA9 genes and an increase in H+ ATPase enzyme concentration in the plasma membrane. Results from 33P uptake experiments showed that rice roots incubated with NH +4 absorbed more 33P during the four-hour incubation than did rice roots incubated with NO -3 . Vanadate inhibited 33P uptake in rice roots supplied with NH +4 , while fusicoccin stimulated 33P uptake under NO -3 nutrition.

Conclusions

Taken together, these results suggest an involvement of plasma membrane H+ ATPase in the stimulated uptake of phosphorus by rice roots supplemented with NH +4 .

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Acknowledgements

This work was supported by Natural Science Foundation of China (NSFC 30971864).

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Authors and Affiliations

  1. College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China

    Houqing Zeng, Gan Liu, Ruiping Zhang, Yiyong Zhu, Qirong Shen & Guohua Xu

  2. Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan

    Toshinori Kinoshita

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  1. Houqing Zeng
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  2. Gan Liu
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  3. Toshinori Kinoshita
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  4. Ruiping Zhang
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  5. Yiyong Zhu
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  6. Qirong Shen
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  7. Guohua Xu
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Correspondence to Yiyong Zhu.

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Responsible Editor: Michael Denis Cramer.

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Zeng, H., Liu, G., Kinoshita, T. et al. Stimulation of phosphorus uptake by ammonium nutrition involves plasma membrane H+ ATPase in rice roots. Plant Soil 357, 205–214 (2012). https://doi.org/10.1007/s11104-012-1136-4

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