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Biological control of pathogen communication in the rhizosphere: A novel approach applied to potato soft rot due to Pectobacterium atrosepticum

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Abstract

Background and aims

Recent basic knowledge on the regulation of virulence in pectinolytic bacteria revealed pathogen communication via quorum sensing signals as a crucial event for the expression of virulence and the onset of disease symptoms. In this paper, we present and discuss advances in a new biocontrol approach based on the interference of microbial communication involved in the cellular density and microenvironment sensoring.

Methods

This emerging strategy consists in the characterization of the signaling molecules used by the target pathogen, then the use of harmless structural analogs to stimulate plant associated-microflora able to degrade both molecule families.

Results

The biocontrol method has been applied for the first time for the control of Pectobacterium atrosepticum. This psychrotrophic bacterium synthesizes N-acyl-homoserine lactones involved in cell-to-cell communication that triggers soft rot and blackleg of potato. The use of the gamma-caprolactone stimulant promotes the emergence and catabolic activity of Rhodococcus erythropolis antagonistic populations in the potato rhizosphere.

Conclusions

Rhodococcus bacteria have the ability to disrupt the quorum sensing-based communication of P. atrosepticum by degrading N-acyl-homoserine lactone signaling molecules and prevent disease.

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Acknowledgments

ACr and ACi are supported by Comité Nord Plants de Pommes de terre (CNPPT, France) and ANRT (CIFRE) ; CB, NO, JFB, and XL are funded by grants from Conseil Régional de Haute-Normandie & Ministère délégué à l’Enseignement Supérieur et à la Recherche, GRR VATA & FEDER, and by the national grant CAS-DAR AAP N°7124; MT, YD, and DF are funded by CNRS, CNRS program Ingénierie Ecologique, National program PESTICIDES, and Région Ile-de-France (DIM R2DS). Works are related to COST631 action-Understanding and Modelling Plant-Soil Interactions in the Rhizosphere Environment. We also thank Christine Farmer for linguistic support.

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Authors and Affiliations

  1. Laboratory of Microbiology Signals and Microenvironment—Normandy University—EA 4312 University of Rouen, IUT Evreux 55 rue Saint-Germain, F-27000, Evreux, France

    Alexandre Crépin, Corinne Barbey, Nicole Orange, Marc Feuilloley, Jean-François Burini & Xavier Latour

  2. SPPN—Comité Nord, Research station and potato breeding, 76110, Bretteville du Grand Caux, France

    Alexandre Crépin & Amélie Cirou

  3. CNRS, Institute of Plant Sciences UPR2355, 1 Avenue de la Terrasse, 91198, Gif-sur-Yvette, France

    Amélie Cirou, Mélanie Tannières, Yves Dessaux & Denis Faure

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  1. Alexandre Crépin
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  2. Corinne Barbey
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  3. Amélie Cirou
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  4. Mélanie Tannières
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  5. Nicole Orange
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  6. Marc Feuilloley
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  7. Yves Dessaux
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  8. Jean-François Burini
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  9. Denis Faure
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  10. Xavier Latour
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Corresponding authors

Correspondence to Denis Faure or Xavier Latour.

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Responsible Editor: Bernard Glick.

Alexandre Crépin and Corinne Barbey contributed equally to this paper.

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Crépin, A., Barbey, C., Cirou, A. et al. Biological control of pathogen communication in the rhizosphere: A novel approach applied to potato soft rot due to Pectobacterium atrosepticum . Plant Soil 358, 27–37 (2012). https://doi.org/10.1007/s11104-011-1030-5

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