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Transcript responses in leaves of ozone-treated beech saplings seasons at an outdoor free air model fumigation site over two growing seasons

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Abstract

The still most abundant air pollutant, tropospheric ozone, leads to severe oxidative stress in plants. To investigate the ozone responsiveness of trees, we carried out a microarray analysis of beech saplings, grown around a free air lysimeter station in the years 2005 and 2006. The microarrays revealed ozone-responsive expressed sequence tags (ESTs), derived from a suppression subtractive hybridisation cDNA library. Chronic ozone exposure effects were investigated at different time points throughout the whole vegetation period in 2005 and 2006. Significantly regulated ESTs were calculated with R-packages. Different transcript patterns in both years could be related to weather conditions and consequential a different ozone uptake. Fewer transcriptome changes were found in 2005 compared to 2006. Induction of ESTs involved in cell structure, related to stress response and cell walls, signal transduction, as well as disease and defence in July and August indicated ozone-related stress responses in both years. An early response to ozone on primary metabolism, or, more precisely, on plant growth and photosynthesis, was observed in July and August particularly in the year 2006. This study demonstrated a clear transcriptional ozone response of juvenile beech, the most important deciduous tree in Central Europe, under free air conditions.

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Acknowledgements

We wish to thank the technical staff of the Department for Environmental Engineering for their excellent assistance and our colleague Werner Heller for critical reading the manuscript. This work was supported by the Deutsche Forschungsgemeinschaft (SFB 607) and in part by the European Community (Evoltree, 6th Framework Programme; COST Action E52).

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Authors and Affiliations

  1. Helmholtz Zentrum München – German Research Center for Environmental Health, Institute of Biochemical Plant Pathology, 85764, Neuherberg, Germany

    Maren Olbrich, Elke Gerstner & Dieter Ernst

  2. Helmholtz Zentrum München – German Research Center for Environmental Health, Institute of Developmental Genetics, 85764, Neuherberg, Germany

    Gerhard Welzl

  3. Helmholtz Zentrum München – German Research Center for Environmental Health, Institute of Soil Ecology, Department of Environmental Engineering, 85764, Neuherberg, Germany

    J. Barbro Winkler

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  1. Maren Olbrich
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  2. Elke Gerstner
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  3. Gerhard Welzl
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  4. J. Barbro Winkler
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  5. Dieter Ernst
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Correspondence to Maren Olbrich.

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Responsible Editor: Yong Chao Liang.

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Table S1

Number of ozone-responsive unknown ESTs. (DOC 29 kb)

Table S2

Regulation of gene expression for significantly regulated genes in European beech grown under twice ambient ozone. (DOC 36 kb)

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Olbrich, M., Gerstner, E., Welzl, G. et al. Transcript responses in leaves of ozone-treated beech saplings seasons at an outdoor free air model fumigation site over two growing seasons. Plant Soil 323, 61–74 (2009). https://doi.org/10.1007/s11104-009-0129-4

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