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Ozone-induced changes of mRNA levels of β-1,3-glucanase, chitinase and ‘pathogenesis-related’ protein 1b in tobacco plants

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Abstract

Treatment of the ozone-sensitive tobacco cultivar Bel W3 with an ozone pulse (0.15 μl/l, 5 h) markedly increased the mRNA level of basic β-1,3-glucanase and to a lower degree that of basic chitinase. The increase of β-1,3-glucanase mRNA level occurred within 1 h and showed a transient maximum. Seventeen hours after ozone treatment, the β-1,3-glucanase mRNA level decreased to lower values. The increase of basic chitinase mRNA level was delayed and was less pronounced than that of β-1,3-glucanase mRNA. Cultivar Bel B showed only a small increase of β-1,3-glucanase mRNA level after the same ozone treatment, whereas its basic chitinase mRNA was more strongly induced. Prolonged ozone treatment for 2 days of tobacco Bel W3 led to a persistent level of β-1,3-glucanase and basic chitinase mRNAs, as well as to an increase of acidic chitinase and ‘pathogenesis-related’ (PR) 1b mRNA levels. The results indicate that genes so far considered to code for PR proteins may also be involved in the plant response to oxidative stress.

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Authors and Affiliations

  1. GSF-Forschungszentrum für Umwelt und Gesundheit GmbH, Institut für Biochemische Pflanzenpathologie, D-8042, Neuherberg, FRG

    D. Ernst, M. Schraudner, C. Langebartels & H. Sandermann Jr.

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  1. D. Ernst
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  2. M. Schraudner
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  3. C. Langebartels
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  4. H. Sandermann Jr.
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Ernst, D., Schraudner, M., Langebartels, C. et al. Ozone-induced changes of mRNA levels of β-1,3-glucanase, chitinase and ‘pathogenesis-related’ protein 1b in tobacco plants. Plant Mol Biol 20, 673–682 (1992). https://doi.org/10.1007/BF00046452

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  • DOI: https://doi.org/10.1007/BF00046452

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