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Effect of co-inoculation of methylotrophic Methylobacterium oryzae with Azospirillum brasilense and Burkholderia pyrrocinia on the growth and nutrient uptake of tomato, red pepper and rice

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Abstract

The present greenhouse study was undertaken to evaluate the effects of co-inoculating methylotrophic Methylobacterium oryzae CBMB20 along with nitrogen-fixing Azospirillum brasilense CW903 or a phosphate solubilizing bacterium Burkholderia pyrrocinia CBPB-HOD on the growth and nutrient uptake of tomato, red pepper and rice. Seed inoculation and soil/foliar application of the bacterial strains alone or under dual inoculation increased the plant growth in terms of shoot or root length and increased the nutrient uptake in the plants studied compared to uninoculated control plants. Co-inoculation of M. oryzae CBMB20 with A. brasilense CW903 or B. pyrrocinia CBPB-HOD improved the N and P concentration of plants, while the results varied among the plant species tested. Also, co-inoculation of the bacterial strains increased the activity of nitrogenase, urease and phosphatase enzymes in soil when compared to uninoculated control or individual inoculations. Though the inoculation effects were analyzed at an early stage of plant growth, the results conclusively suggest that M. oryzae being compatible with other microorganisms in the rhizosphere can potentially be used as individual inoculant or co-inoculated with other plant growth promoting bacteria to increase the production in sustainable agricultural systems.

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Acknowledgements

This study was carried out with the support of “Cooperative Research Program for Agricultural Science and Technology Development” Rural Development Administration (RDA), Republic of Korea. The authors also thank Soo-Jae Park and Sook-Jin Kim for their help and assistance in conducting the pot culture experiments and for analyzing the samples by ICP-OES.

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  1. M. Madhaiyan

    Present address: Biomaterials and Biocatalysts Group, Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore, 117604, Singapore

Authors and Affiliations

  1. Department of Agricultural Chemistry, Chungbuk National University, Cheongju, Chungbuk, 361-763, Republic of Korea

    M. Madhaiyan, S. Poonguzhali & Tong-Min Sa

  2. Protected Agricultural Experiment Institute, CBARES, Umseong, 369-824, Republic of Korea

    Bo-Goo Kang

  3. National Institute of Agricultural Sciences and Technology, Rural Development Administration, Suwon, 441-707, Republic of Korea

    Yun-Jeong Lee

  4. Division of Life and Environmental Sciences, Daegu University, Daegu, Gyeongsan, 712-714, Republic of Korea

    Jong-Bae Chung

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  1. M. Madhaiyan
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Correspondence to M. Madhaiyan.

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Responsible Editor: Jesus Mercado-Blanco.

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Madhaiyan, M., Poonguzhali, S., Kang, BG. et al. Effect of co-inoculation of methylotrophic Methylobacterium oryzae with Azospirillum brasilense and Burkholderia pyrrocinia on the growth and nutrient uptake of tomato, red pepper and rice. Plant Soil 328, 71–82 (2010). https://doi.org/10.1007/s11104-009-0083-1

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