Abstract
It is an intriguing issue of evolutionary biology how genetic diversity and gene expression diversity shape the adaptive patterns. Comparative transcriptomic studies of wild populations in extreme environments provide critical insights into the relative contribution of genetic and expressive components. In this study, we analyzed the genetic diversity and gene expression diversity of 20 populations of the aquatic plant Batrachium bungei along elevations ranging from 2690 to 4896 m on the Qinghai–Tibet plateau (QTP). Based on single nucleotide polymorphisms (SNPs) and gene expression data from 100 individuals of B. bungei, we found that variation in genetic sequence was more sensitive to detect weak differentiation than gene expression. Using 292,613 high-quality SNPs, we documented a significant phylogeographical structure, a low within-population genetic diversity, and a high inter-population genetic differentiation in B. bungei populations. Analysis of relationship between geographic distance, genetic distance, and gene expression similarity showed that geographic isolation shaped gene flow patterns but not gene expression patterns. We observed a negative relationship between genetic diversity and gene expression diversity within and among B. bungei populations, and we demonstrated that as environmental conditions worsen with increasing altitude, genetic diversity played an increased role in maintaining the stability of populations, while the corresponding role of gene expression diversity decreased. These results suggested that genetic diversity and gene expression diversity might act as a complementary mechanism contributing to the long-term survival of B. bungei in extreme environments.
Key message
Genetic diversity and gene expression diversity might act as a complementary mechanism contributing to the long-term survival of B. bungei in extreme environments.
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Data availability
The RNA sequencing data generated in this work have been deposited to the NCBI’s Sequence Read Archive under accession number SRR18512371-SRR18512470 (BioProject PRJNA820952) (https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA820952).
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Acknowledgements
This work was supported in part by the National Natural Science Foundation of China (31860046), and Plateau Ecology Youth Innovative Fund of Wuhan University (413100105). We sincerely thank Wenkai Li from China University of Geoscience for his critical help on data visualization. Additionally, we also thank Liya Ji, Guoqi Xu, Xuanfeng Wu, Xinzhong Li and Pincuo Zhaxi from Tibet University for their critical assistance on sample collection.
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Funding was provided by National Natural Science Foundation of China (Grant No. 31860046).
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XL and XL conceived the study. XY completed the data analysis with the help of FC, ZC, PW, CL and XS helped with the data visualization. TL and FC collected the samples, and XY wrote the manuscript with the help of XL.
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Yu, X., Chen, F., Chen, Z. et al. Genetic diversity and gene expression diversity shape the adaptive pattern of the aquatic plant Batrachium bungei along an altitudinal gradient on the Qinghai–Tibet plateau. Plant Mol Biol 111, 275–290 (2023). https://doi.org/10.1007/s11103-022-01326-0
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DOI: https://doi.org/10.1007/s11103-022-01326-0