Abstract
Key message
Os4BGlu14, a monolignol β-glucosidase, plays a negative role in seed longevity by affecting primary metabolism during seed development and aging.
Abstract
Seed longevity is a crucial trait in agriculture and in the conservation of germplasm resources. β-Glucosidases (BGlus) are multifunctional enzymes that affect plant growth and their adaptation to the environment. The function of rice BGlus in seed longevity, however, remains unknown. We report here that Os4BGlu14, a rice β-Glucosidase, negatively affected seed longevity during accelerated aging. Os4BGlu14 was highly expressed in rice embryos and induced by accelerated aging. Compared to the wild type, rice lines overexpressing Os4BGlu14 had significantly greater grain length, but smaller grain width and thickness. Overexpressing (OE) lines also showed lower starch but higher glucose contents. After accelerated aging treatment, OE lines displayed a significantly lower germination percentage than the wild type. Additionally, these lines had higher lignin accumulation before and after accelerated aging. Metabolome analysis detected 217 metabolites in untreated and aged rice seeds. Comparison of the differential metabolites between WT and OE5 revealed that ten key metabolites, four of which (e.g., uridine 5′-diphosphoglucose-glucose, UDPG) were increased, while the other six (e.g., γ-aminobutyric acid and methionine) were decreased, might be the crucial factors that lead to seed deterioration. Further analysis confirmed higher UDPG levels and more severe programmed cell death in OE lines than in the wild type. Furthermore, OE lines presented a lower germination rate after abscisic acid and paclobutrazol treatment during germination, compared to the wild type. Our study provides a basis for understanding the function of Os4BGlu14 in seed longevity in rice.
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Funding
This study was funded by the grants of the National Natural Science Foundation of China (No. 31870304, and No. 31571760), the key program of the Natural Science Foundation of Tianjin (No. 18JCZDJC33700, and No.17JCZDJC34000) and the creative group project of the Rice Industry Technological System of Tianjin (No. ITTRRS2018005).
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XWC and DFC designed and advised the study. RJR, PW, LNW, LJS and YS performed the experiments. RJR, PW, JPS, DFC and XWC analyzed the data. RJR, DFC and XWC wrote the paper. All authors read and approved the manuscript.
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Ren, RJ., Wang, P., Wang, LN. et al. Os4BGlu14, a monolignol β-Glucosidase, negatively affects seed longevity by influencing primary metabolism in rice. Plant Mol Biol 104, 513–527 (2020). https://doi.org/10.1007/s11103-020-01056-1
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DOI: https://doi.org/10.1007/s11103-020-01056-1