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Increased fes1a thermotolerance is induced by BAG6 knockout

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Abstract

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(1) The fes1a bag6 double mutant shows an increased short term thermotolerance compared to fes1a. BAG6 is a suppressor of Fes1A; (2) IQ motif is essential to effective performance of BAG6. (3) Calmodulin was involved in signal transduction. (4) BAG6 is localized in the nucleus.

Abstract

HSP70s play an important role in the heat-induced stress tolerance of plants. However, effective HSP70 function requires the assistance of many co-chaperones. BAG6 and Fes1A are HSP70-binding proteins that are critical for Arabidopsis thaliana thermotolerance. Despite this importance, little is known about how these co-chaperones interact. In this study, we assessed the thermotolerance of a fes1a bag6 double mutant. We found that the fes1a bag6 double mutant shows an increased short-term thermotolerance compared to fes1a. However, calmodulin inhibitors diminished this enhanced thermotolerance in the fes1a bag6 double mutant. In addition, we found the IQ motif to be essential for effective BAG6 performance. Since BAG6 is localized in the nucleus, the signal transduction is likely to involve nuclear calcium signaling.

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Acknowledgements

The National Natural Science Foundation of China (Grant No. 31270298) and Research Fundamental Capacity Improvement Project for Middle Age and Youth Teachers of Guangxi Universities (Grant No. 2019KY0517) supported this study.

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Author notes

  1. Can Fu and Yanfei Hou have contributed equally to this work.

Authors and Affiliations

  1. College of Life Science, Shandong Normal University, Jinan, 250014, Shandong, China

    Can Fu, Yanfei Hou, Jingjing Ge, Limin Zhang, Xiaxia Liu, Panfei Huo & Jian Liu

  2. College of Biotechnology, Guilin Medical University, Guilin, 541004, Guangxi, China

    Can Fu

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  1. Can Fu
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  2. Yanfei Hou
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  3. Jingjing Ge
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  7. Jian Liu
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Contributions

CF carried out the experiment with YH, LZ, XL, and PH, and wrote the main manuscript text. YH and JG supplemented the experiments that the reviewers suggested to improve the manuscript. JL designed and supervised the research and revised the manuscript. All authors reviewed the manuscript.

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Correspondence to Jian Liu.

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Fu, C., Hou, Y., Ge, J. et al. Increased fes1a thermotolerance is induced by BAG6 knockout. Plant Mol Biol 100, 73–82 (2019). https://doi.org/10.1007/s11103-019-00844-8

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